Use of in vitro kernel culture to study maize nitrogen and carbohydrate metabolism

Juliann R. Seebauer, Frederick E. Below

Research output: ResearchChapter

Abstract

Grain yield in maize is the result of a genotype’s response to environmental conditions and agronomic management. However, whether in a field, greenhouse, or growth chamber, plant-to-plant variation exists within the same genotype, necessitating large amounts of plants and growth area to determine a metabolic response to a change in growth conditions or fertilizer supply. Additionally, because of whole-plant interactions in the supply of nutrients to kernels, it is difficult to study assimilate or temperature effects on the growth of kernels. The in vitro growth of kernels is one way to circumvent this problem because it allows for kernel growth under defined conditions of nutrient supply, while minimizing environmental and maternal influences. The in vitro kernel culturing method can be used to identify source: sink relationships, assimilate transport, metabolism, plant growth regulators, and other physiological responses by altering the source supply to individual kernels within an ear, thereby reducing or controlling environmental effects, while maintaining kernel-cob and organ-wide interactions. A single control-pollinated immature maize ear can be divided and quickly subjected to various growth conditions using liquid media to more precisely generate physiological and metabolic changes in the earshoot than in planta.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages3-13
Number of pages11
Volume1676
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1676
ISSN (Print)1064-3745

Keywords

  • Assimilates
  • Grain
  • In vitro
  • Kernel
  • Maize
  • Metabolism
  • Ovule
  • Physiology
  • Transport

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Seebauer, J. R., & Below, F. E. (2018). Use of in vitro kernel culture to study maize nitrogen and carbohydrate metabolism. In Methods in Molecular Biology (Vol. 1676, pp. 3-13). (Methods in Molecular Biology; Vol. 1676). Humana Press Inc.. DOI: 10.1007/978-1-4939-7315-6_1

Use of in vitro kernel culture to study maize nitrogen and carbohydrate metabolism. / Seebauer, Juliann R.; Below, Frederick E.

Methods in Molecular Biology. Vol. 1676 Humana Press Inc., 2018. p. 3-13 (Methods in Molecular Biology; Vol. 1676).

Research output: ResearchChapter

Seebauer, JR & Below, FE 2018, Use of in vitro kernel culture to study maize nitrogen and carbohydrate metabolism. in Methods in Molecular Biology. vol. 1676, Methods in Molecular Biology, vol. 1676, Humana Press Inc., pp. 3-13. DOI: 10.1007/978-1-4939-7315-6_1
Seebauer JR, Below FE. Use of in vitro kernel culture to study maize nitrogen and carbohydrate metabolism. In Methods in Molecular Biology. Vol. 1676. Humana Press Inc.2018. p. 3-13. (Methods in Molecular Biology). Available from, DOI: 10.1007/978-1-4939-7315-6_1
Seebauer, Juliann R. ; Below, Frederick E./ Use of in vitro kernel culture to study maize nitrogen and carbohydrate metabolism. Methods in Molecular Biology. Vol. 1676 Humana Press Inc., 2018. pp. 3-13 (Methods in Molecular Biology).
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