Use of green fluorescent protein and reverse transcription-PCR to monitor Candida albicans agglutinin-like sequence gene expression in a murine model of disseminated candidiasis

Clayton B. Green, Xiaomin Zhao, Lois L. Hoyer

Research output: Contribution to journalArticlepeer-review

Abstract

Candida albicans PALS-green fluorescent protein (GFP) reporter strains were inoculated into mice in a disseminated candidiasis model, and GFP production was monitored by immunohistochemistry and reverse transcription-PCR (RT-PCR). GFP production from the ALS1 and ALS3 promoters was detected immunohistochemically. ALS1, ALS2, ALS3, ALS4, and ALS9 transcription was detected by RT-PCR, further identifying ALS genes expressed in this model.

Original languageEnglish (US)
Pages (from-to)1852-1855
Number of pages4
JournalInfection and immunity
Volume73
Issue number3
DOIs
StatePublished - Mar 2005

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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