Use of biotin-labeled probes to map specific dna sequences on wheat chromosomes

A. L. Rayburn, B. S. Gill

Research output: Contribution to journalArticlepeer-review

Abstract

A biotin-labeling technique was used to map a 120 bp rye DNA probe by in situ hybridization to somatic metaphase chromosomes of common wheat. Twenty-four hybridization sites were revealed on 11 chromosomes including 4A, 2D, 3D, 5D, and the seven B-genome chromosomes. The observed results were similar to those of previous studies that used isotope labeling. Biotin labeling was found to be a rapid, consistent, and reliable technique to detect repeated DNA sequences by in situ hybridization in wheat and should be a useful technique for the physical mapping of DNA sequences on plant chromosomes.

Original languageEnglish (US)
Pages (from-to)78-81
Number of pages4
JournalJournal of Heredity
Volume76
Issue number2
DOIs
StatePublished - Mar 1985
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Fingerprint Dive into the research topics of 'Use of biotin-labeled probes to map specific dna sequences on wheat chromosomes'. Together they form a unique fingerprint.

Cite this