Use of adrenocorticotropin challenges to indicate chronic stress responses of laying hens in several housing alternatives

Kenneth W Koelkebeck, J. R. Cain, M. S. Amoss

Research output: Contribution to journalArticle

Abstract

The use of adrenocorticotropin (ACTH) challenges was tested as an indicator of the level of physiological stress response of laying hens in several housing alternatives. Single Comb White Leghorn (SCWL) hens were challenged with intravenous ACTH injections (either .33, 1.0 or 3.0 IU/kg body weight). Plasma corticosterone (CORT) concentrations taken at intervals from 10 to 180 min following ACTH challenges revealed CORT response curves which peaked within 30 min at 34-, 54- and 60-fold increases over pre-challenge CORT levels with doses of .33, 1.0 and 3.0 IU/kg ACTH, respectively. Peak CORT levels for 1.0 and 3.0 IU/kg of ACTH did not differ; however, CORT levels for 1.0 IU/kg ACTH were significantly elevated above the peak for .33 IU/kg ACTH. Laying hens housed in cage and floor management alternatives were challenged with .33 and 1.0 IU/kg of ACTH after 10 months of lay. Cage management treatments were 3, 4 or 5 hens per cage and floor treatments with hens kept at 2 densities. Significant differences in the ACTH-induced CORT response between management alternatives could be detected following injection of .33 IU/kg ACTH, but not with the higher ACTH dose (1.0 IU/kg). These studies demonstrated that the CORT response to the lower dose of ACTH seemed to be more effective in assessing management differences, but further clarification may be necessary before ACTH challenges can be accurately used to evaluate physiological stress responses of laying hens housed in different conditions.

Original languageEnglish (US)
Pages (from-to)301-305
Number of pages5
JournalDomestic Animal Endocrinology
Volume3
Issue number4
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

Fingerprint

corticotropin
Adrenocorticotropic Hormone
laying hens
stress response
corticosterone
Corticosterone
hens
cages
Physiological Stress
dosage
injection
Comb and Wattles
White Leghorn
Intravenous Injections

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology
  • veterinary(all)

Cite this

Use of adrenocorticotropin challenges to indicate chronic stress responses of laying hens in several housing alternatives. / Koelkebeck, Kenneth W; Cain, J. R.; Amoss, M. S.

In: Domestic Animal Endocrinology, Vol. 3, No. 4, 01.01.1986, p. 301-305.

Research output: Contribution to journalArticle

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