Use of a head-to-tail peptide cyclase to prepare hybrid RiPPs

Tung Le, Dongtianyu Zhang, Rachel M. Martini, Subhanip Biswas, Wilfred A. van der Donk

Research output: Contribution to journalArticlepeer-review


Cyclotides and lanthipeptides are cyclic peptide natural products with promising bioengineering potential. No peptides have been isolated that contain both structural motifs defining these two families, an N-to-C cyclised backbone and lanthionine linkages. We combined their biosynthetic machineries to produce hybrid structures that possess improved activity or stability, demonstrate how the AEP-1 plant cyclase can be utilised to complete the maturation of the sactipeptide subtilosin A, and present head-to-tail cyclisation of the glycocin sublancin. These studies show the plasticity of AEP-1 and its utilisation alongside other post-translational modifications.
Original languageEnglish (US)
Pages (from-to)6508-6511
Number of pages4
JournalChemical Communications
Issue number51
StatePublished - Jun 4 2024


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