Urinary bisphenol A concentrations and cytochrome P450 19 A1 (Cyp19) gene expression in ovarian granulosa cells: An in vivo human study

Shelley Ehrlich; Paige L. Williams; Russ Hauser; Stacey A. Missmer; Jackye Peretz; Antonia M. Calafat; Jodi A. Flaws

doi:10.1016/j.reprotox.2013.06.071

Urinary bisphenol A concentrations and cytochrome P450 19 A1 (Cyp19) gene expression in ovarian granulosa cells: An in vivo human study

Shelley Ehrlich, Paige L. Williams, Russ Hauser, Stacey A. Missmer, Jackye Peretz, Antonia M. Calafat, Jodi A. Flaws

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Exposure to bisphenol A (BPA), a chemical widely used in consumer products, has been associated with in vitro Cyp19 gene expression. Objective: To evaluate an in vivo human model of Cyp19 gene expression in granulosa cells. Study Design: A subset of an ongoing prospective cohort study of women undergoing in vitro fertilization (IVF) at Massachusetts General Hospital. Methods: Mixed effect models were used to evaluate the association of urinary BPA concentrations with granulosa cell Cyp19 mRNA expression. Results: In 61 women undergoing 76 IVF cycles, adjusted changes in mean Cyp19 expression (β estimate (95% CI)) for quartiles 2, 3 and 4 as compared to the lowest quartile were: -0.97 (-2.22, 0.28); -0.97 (-2.18, 0.24) and -0.38 (-1.58, 0.82). Conclusions: An in vivo model for evaluation of Cyp19 gene expression was developed for use in epidemiologic studies. In this pilot study, we found no statistically significant linear association between urinary BPA concentrations and Cyp19 expression.

Original language	English (US)
Pages (from-to)	18-23
Number of pages	6
Journal	Reproductive Toxicology
Volume	42
DOIs	https://doi.org/10.1016/j.reprotox.2013.06.071
State	Published - Dec 2013

Keywords

Bisphenol A
Cyp19
Fertility
Follicular fluid
Gene expression
Human
Ovary, granulosa cells
P450 aromatase

ASJC Scopus subject areas

Toxicology

Online availability

10.1016/j.reprotox.2013.06.071

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@article{05870ccb54a3404cae75aaa3a873b908,

title = "Urinary bisphenol A concentrations and cytochrome P450 19 A1 (Cyp19) gene expression in ovarian granulosa cells: An in vivo human study",

abstract = "Background: Exposure to bisphenol A (BPA), a chemical widely used in consumer products, has been associated with in vitro Cyp19 gene expression. Objective: To evaluate an in vivo human model of Cyp19 gene expression in granulosa cells. Study Design: A subset of an ongoing prospective cohort study of women undergoing in vitro fertilization (IVF) at Massachusetts General Hospital. Methods: Mixed effect models were used to evaluate the association of urinary BPA concentrations with granulosa cell Cyp19 mRNA expression. Results: In 61 women undergoing 76 IVF cycles, adjusted changes in mean Cyp19 expression (β estimate (95% CI)) for quartiles 2, 3 and 4 as compared to the lowest quartile were: -0.97 (-2.22, 0.28); -0.97 (-2.18, 0.24) and -0.38 (-1.58, 0.82). Conclusions: An in vivo model for evaluation of Cyp19 gene expression was developed for use in epidemiologic studies. In this pilot study, we found no statistically significant linear association between urinary BPA concentrations and Cyp19 expression.",

keywords = "Bisphenol A, Cyp19, Fertility, Follicular fluid, Gene expression, Human, Ovary, granulosa cells, P450 aromatase",

author = "Shelley Ehrlich and Williams, {Paige L.} and Russ Hauser and Missmer, {Stacey A.} and Jackye Peretz and Calafat, {Antonia M.} and Flaws, {Jodi A.}",

note = "Funding Information: This work was supported by grant ES009718 , ES000002 , ES019178 from the National Institute of Environmental Health Sciences and grant OH008578 from the National Institute for Occupational Safety and Health . ",

year = "2013",

month = dec,

doi = "10.1016/j.reprotox.2013.06.071",

language = "English (US)",

volume = "42",

pages = "18--23",

journal = "Reproductive Toxicology",

issn = "0890-6238",

publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Urinary bisphenol A concentrations and cytochrome P450 19 A1 (Cyp19) gene expression in ovarian granulosa cells

T2 - An in vivo human study

AU - Ehrlich, Shelley

AU - Williams, Paige L.

AU - Hauser, Russ

AU - Missmer, Stacey A.

AU - Peretz, Jackye

AU - Calafat, Antonia M.

AU - Flaws, Jodi A.

N1 - Funding Information: This work was supported by grant ES009718 , ES000002 , ES019178 from the National Institute of Environmental Health Sciences and grant OH008578 from the National Institute for Occupational Safety and Health .

PY - 2013/12

Y1 - 2013/12

N2 - Background: Exposure to bisphenol A (BPA), a chemical widely used in consumer products, has been associated with in vitro Cyp19 gene expression. Objective: To evaluate an in vivo human model of Cyp19 gene expression in granulosa cells. Study Design: A subset of an ongoing prospective cohort study of women undergoing in vitro fertilization (IVF) at Massachusetts General Hospital. Methods: Mixed effect models were used to evaluate the association of urinary BPA concentrations with granulosa cell Cyp19 mRNA expression. Results: In 61 women undergoing 76 IVF cycles, adjusted changes in mean Cyp19 expression (β estimate (95% CI)) for quartiles 2, 3 and 4 as compared to the lowest quartile were: -0.97 (-2.22, 0.28); -0.97 (-2.18, 0.24) and -0.38 (-1.58, 0.82). Conclusions: An in vivo model for evaluation of Cyp19 gene expression was developed for use in epidemiologic studies. In this pilot study, we found no statistically significant linear association between urinary BPA concentrations and Cyp19 expression.

AB - Background: Exposure to bisphenol A (BPA), a chemical widely used in consumer products, has been associated with in vitro Cyp19 gene expression. Objective: To evaluate an in vivo human model of Cyp19 gene expression in granulosa cells. Study Design: A subset of an ongoing prospective cohort study of women undergoing in vitro fertilization (IVF) at Massachusetts General Hospital. Methods: Mixed effect models were used to evaluate the association of urinary BPA concentrations with granulosa cell Cyp19 mRNA expression. Results: In 61 women undergoing 76 IVF cycles, adjusted changes in mean Cyp19 expression (β estimate (95% CI)) for quartiles 2, 3 and 4 as compared to the lowest quartile were: -0.97 (-2.22, 0.28); -0.97 (-2.18, 0.24) and -0.38 (-1.58, 0.82). Conclusions: An in vivo model for evaluation of Cyp19 gene expression was developed for use in epidemiologic studies. In this pilot study, we found no statistically significant linear association between urinary BPA concentrations and Cyp19 expression.

KW - Bisphenol A

KW - Cyp19

KW - Fertility

KW - Follicular fluid

KW - Gene expression

KW - Human

KW - Ovary, granulosa cells

KW - P450 aromatase

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Urinary bisphenol A concentrations and cytochrome P450 19 A1 (Cyp19) gene expression in ovarian granulosa cells: An in vivo human study

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