Uptake, distribution and toxicity of gold nanoparticles in tobacco (Nicotiana xanthi) seedlings

Tara Sabo-Attwood, Jason M. Unrine, John W. Stone, Catherine J. Murphy, Soumitra Ghoshroy, Doug Blom, Paul M. Bertsch, Lee A. Newman

Research output: Contribution to journalArticlepeer-review

Abstract

Understanding plant interactions with nanoparticles is of increasing importance for assessing their toxicity and trophic transport. The primary objective of this study was to assess uptake, biodistribution and toxicity associated with exposure of tobacco plants (Nicotiana xanthi) to gold nanoparticles (AuNPs). We employed synchrotron-based X-ray microanalysis with X-ray absorption near-edge microspectroscopy and high resolution electron microscopy to localize AuNPs within plants. Results from these experiments reveal that AuNPs entered plants through the roots and moved into the vasculature. Aggregate bodies were also detected within root cell cytoplasm. Furthermore, AuNP uptake was size selective as 3.5 nm AuNP spheres were detected in plants but 18 nm AuNPs remained agglomerated on the root outer surfaces. Finally, leaf necrosis was observed after 14 days of exposure to 3.5 nm AuNPs. Overall, results of this work show the potential for AuNPs to enter plants through size-dependent mechanisms, translocate to cells and tissues and cause biotoxicity.

Original languageEnglish (US)
Pages (from-to)353-360
Number of pages8
JournalNanotoxicology
Volume6
Issue number4
DOIs
StatePublished - Jun 2012
Externally publishedYes

Keywords

  • Gold nanoparticles
  • Nicotiana xanthi
  • Spectroscopy
  • XANES

ASJC Scopus subject areas

  • Biomedical Engineering
  • Toxicology

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