TY - JOUR
T1 - Upconversion Luminescence-Activated DNA Nanodevice for ATP Sensing in Living Cells
AU - Zhao, Jian
AU - Gao, Jinhong
AU - Xue, Wenting
AU - Di, Zhenghan
AU - Xing, Hang
AU - Lu, Yi
AU - Li, Lele
N1 - Publisher Copyright:
© 2017 American Chemical Society.
PY - 2018/1/17
Y1 - 2018/1/17
N2 - Designer DNA nanodevices have attracted extensive interest for detection of specific targets in living cells. However, it still remains a great challenge to construct DNA sensing devices that can be activated at desired time with a remotely applied stimulus. Here we report a rationally designed, synthetic DNA nanodevice that can detect ATP in living cells in an upconversion luminescence-activatable manner. The nanodevice consists of a UV light-activatable aptamer probe and lanthanide-doped upconversion nanoparticles which acts as the nanotransducers to operate the device in response to NIR light. We demonstrate that the nanodevice not only enables efficient cellular delivery of the aptamer probe into live cells, but also allows the temporal control over its fluorescent sensing activity for ATP by NIR light irradiation in vitro and in vivo. Ultimately, with the availability of diverse aptamers selected in vitro, the DNA nanodevice platform will allow NIR-triggered sensing of various targets as well as modulation of biological functions in living systems.
AB - Designer DNA nanodevices have attracted extensive interest for detection of specific targets in living cells. However, it still remains a great challenge to construct DNA sensing devices that can be activated at desired time with a remotely applied stimulus. Here we report a rationally designed, synthetic DNA nanodevice that can detect ATP in living cells in an upconversion luminescence-activatable manner. The nanodevice consists of a UV light-activatable aptamer probe and lanthanide-doped upconversion nanoparticles which acts as the nanotransducers to operate the device in response to NIR light. We demonstrate that the nanodevice not only enables efficient cellular delivery of the aptamer probe into live cells, but also allows the temporal control over its fluorescent sensing activity for ATP by NIR light irradiation in vitro and in vivo. Ultimately, with the availability of diverse aptamers selected in vitro, the DNA nanodevice platform will allow NIR-triggered sensing of various targets as well as modulation of biological functions in living systems.
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U2 - 10.1021/jacs.7b11161
DO - 10.1021/jacs.7b11161
M3 - Article
C2 - 29281270
AN - SCOPUS:85040645815
SN - 0002-7863
VL - 140
SP - 578
EP - 581
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 2
ER -