Unusual regulatory properties of sucrose‐phosphate synthase purified from soybean (Glycine max) leaves

Tom H. Nielsen, Steven C. Huber

Research output: Contribution to journalArticle

Abstract

Sucrose‐phosphate (SPS) from source leaves of soybean (Glycine max (L.) Merr. cv. Ransom II) was purified 74‐fold to a final specific activity of 1.8 U (mg protein)1. The partially purified preparation was free from phosphoglucoseisomerase (EC 5.3.1.9), pyrophosphatase (EC 3.6.1.1), phosphoenolpyruvate‐phosphatase (EC 3.1.3.‐), phosphofructokinase (EC 2.7.1.11), and uridine diphosphatase (EC 3.6.1.6), and was used for characterization of the kinetic and regulatory properties of the enzyme. The enzyme showed hyperbolic saturation kinetics for both fructose‐6‐phosphate (Km=0.57 mM) and UDPGlucose (UDPG) (Km=4.8 mM). The activity of SPS was inhibited by the product UDP. In vitro this inhibition could be partially overcome by the presence of Mg2+. Inorganic orthophosphate was only slightly inhibitory (35% inhibition at 25 mM phosphate). Glucose‐6‐phosphate (up to 20 mM) had no effect on activity, and did not show any significant interaction with phosphate inhibition. A range of potential effectors was tested and had no effect on SPS activity: Glucose‐1‐phosphate, fructose‐1, 6‐bisphosphate, α‐glycero‐phosphate, dihydroxyacetone‐phosphate, 3‐phosphoglyceric acid, (all at 5 mM), sucrose at 100 mM and pyrophosphate at 0.1 mM. The apparent lack of allosteric regulation of soybean SPS makes this enzyme markedly different from SPS previously characterized from spinach and maize.

Original languageEnglish (US)
Pages (from-to)309-314
Number of pages6
JournalPhysiologia Plantarum
Volume76
Issue number3
DOIs
StatePublished - Jul 1989

Fingerprint

Soybeans
Glycine max
Phosphates
soybeans
phosphoric monoester hydrolases
Enzymes
6-phosphofructokinase
enzymes
glucose-6-phosphate isomerase
inorganic pyrophosphatase
Allosteric Regulation
phosphates
Pyrophosphatases
Glycerophosphates
pyrophosphatases
kinetics
leaves
Phosphofructokinases
phosphofructokinases
Uridine Diphosphate

Keywords

  • Glycine max
  • kinetic and regulatory enzyme properties
  • soybean
  • sucrose‐phosphate synthase

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Unusual regulatory properties of sucrose‐phosphate synthase purified from soybean (Glycine max) leaves. / Nielsen, Tom H.; Huber, Steven C.

In: Physiologia Plantarum, Vol. 76, No. 3, 07.1989, p. 309-314.

Research output: Contribution to journalArticle

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