TY - JOUR
T1 - Unlocking nature's biosynthetic potential by directed genome evolution
AU - Cao, Mingfeng
AU - Tran, Vinh G.
AU - Zhao, Huimin
N1 - Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/12
Y1 - 2020/12
N2 - Microorganisms have been increasingly explored as microbial cell factories for production of fuels, chemicals, drugs, and materials. Among the various metabolic engineering strategies, directed genome evolution has emerged as one of the most powerful tools to unlock the full biosynthetic potential of microorganisms. Here we summarize the directed genome evolution strategies that have been developed in recent years, including adaptive laboratory evolution and various targeted genome-scale engineering strategies, and discuss their applications in basic and applied biological research.
AB - Microorganisms have been increasingly explored as microbial cell factories for production of fuels, chemicals, drugs, and materials. Among the various metabolic engineering strategies, directed genome evolution has emerged as one of the most powerful tools to unlock the full biosynthetic potential of microorganisms. Here we summarize the directed genome evolution strategies that have been developed in recent years, including adaptive laboratory evolution and various targeted genome-scale engineering strategies, and discuss their applications in basic and applied biological research.
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U2 - 10.1016/j.copbio.2020.06.012
DO - 10.1016/j.copbio.2020.06.012
M3 - Review article
C2 - 32721868
AN - SCOPUS:85088634940
SN - 0958-1669
VL - 66
SP - 95
EP - 104
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
ER -