Unexpected effect of the vehicle (grain ethanol) of homeopathic FSH on the in vitro survival and development of isolated ovine preantral follicles

Lartiza F. Lima; Rebeca M.P. Rocha; Ana Beatriz G. Duarte; Ivina R. Brito; Gerlane M. Silva; Giovanna Q. Rodrigues; Diana C.S. Nunes-Pinheiro; Antônia D. Sales; Arlindo A. Moura; Matthew B. Wheeler; Ana Paula R. Rodrigues; Cláudio C. Campello; José Ricardo Figueiredo

doi:10.1002/jemt.22810

Unexpected effect of the vehicle (grain ethanol) of homeopathic FSH on the in vitro survival and development of isolated ovine preantral follicles

Lartiza F. Lima, Rebeca M.P. Rocha, Ana Beatriz G. Duarte, Ivina R. Brito, Gerlane M. Silva, Giovanna Q. Rodrigues, Diana C.S. Nunes-Pinheiro, Antônia D. Sales, Arlindo A. Moura, Matthew B. Wheeler, Ana Paula R. Rodrigues, Cláudio C. Campello, José Ricardo Figueiredo

Research output: Contribution to journal › Article › peer-review

Abstract

The aims of this study were to investigate the effects of medium replacement system (experiment I) and of FSH presentations (homeopathic – FSH 6cH and allopathic FSH – rFSH; experiment II) on the in vitro development, hormone production and gene expression of isolated ovine preantral follicles cultured for 6 days. In experiment I, secondary follicles were cultured in the α-MEM⁺ supplemented with FSH 6cH (0.05 fg/ml) or recombinant bovine FSH (100 ng/ml) without/with daily medium addition. The homeopathic FSH treatments with/without medium addition improved (p <.05) follicular development compared to rFSH100 treatment without addition. FSH 6cH with addition showed the highest (p <.05) estradiol production. To verify whether the effects of homeopathic FSH were not due to its vehicle, experiment II was performed. The α-MEM⁺ was supplemented or not with alcohol (0.2% grain ethanol, v/v), FSH 6cH or rFSH100 with daily medium addition. Surprisingly, we found that all treatments improved follicular development compared to the α-MEM⁺ (p <.05). Moreover, homeopathic FSH was similar to the other treatments including its vehicle. In conclusion, its vehicle (ethanol) causes the effect of homeopathic FSH on in vitro development of isolated ovine preantral follicles.

Original language	English (US)
Pages (from-to)	406-418
Number of pages	13
Journal	Microscopy research and technique
Volume	80
Issue number	4
DOIs	https://doi.org/10.1002/jemt.22810
State	Published - Apr 1 2017

Keywords

ethanol
homeopathy
hormone
in vitro culture
ovarian follicles

ASJC Scopus subject areas

Anatomy
Histology
Instrumentation
Medical Laboratory Technology

Online availability

10.1002/jemt.22810

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Lima, L. F., Rocha, R. M. P., Duarte, A. B. G., Brito, I. R., Silva, G. M., Rodrigues, G. Q., Nunes-Pinheiro, D. C. S., Sales, A. D., Moura, A. A., Wheeler, M. B., Rodrigues, A. P. R., Campello, C. C., & Figueiredo, J. R. (2017). Unexpected effect of the vehicle (grain ethanol) of homeopathic FSH on the in vitro survival and development of isolated ovine preantral follicles. Microscopy research and technique, 80(4), 406-418. https://doi.org/10.1002/jemt.22810

Lima, LF, Rocha, RMP, Duarte, ABG, Brito, IR, Silva, GM, Rodrigues, GQ, Nunes-Pinheiro, DCS, Sales, AD, Moura, AA, Wheeler, MB, Rodrigues, APR, Campello, CC & Figueiredo, JR 2017, 'Unexpected effect of the vehicle (grain ethanol) of homeopathic FSH on the in vitro survival and development of isolated ovine preantral follicles', Microscopy research and technique, vol. 80, no. 4, pp. 406-418. https://doi.org/10.1002/jemt.22810

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abstract = "The aims of this study were to investigate the effects of medium replacement system (experiment I) and of FSH presentations (homeopathic – FSH 6cH and allopathic FSH – rFSH; experiment II) on the in vitro development, hormone production and gene expression of isolated ovine preantral follicles cultured for 6 days. In experiment I, secondary follicles were cultured in the α-MEM+ supplemented with FSH 6cH (0.05 fg/ml) or recombinant bovine FSH (100 ng/ml) without/with daily medium addition. The homeopathic FSH treatments with/without medium addition improved (p <.05) follicular development compared to rFSH100 treatment without addition. FSH 6cH with addition showed the highest (p <.05) estradiol production. To verify whether the effects of homeopathic FSH were not due to its vehicle, experiment II was performed. The α-MEM+ was supplemented or not with alcohol (0.2% grain ethanol, v/v), FSH 6cH or rFSH100 with daily medium addition. Surprisingly, we found that all treatments improved follicular development compared to the α-MEM+ (p <.05). Moreover, homeopathic FSH was similar to the other treatments including its vehicle. In conclusion, its vehicle (ethanol) causes the effect of homeopathic FSH on in vitro development of isolated ovine preantral follicles.",

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AU - Lima, Lartiza F.

AU - Rocha, Rebeca M.P.

AU - Duarte, Ana Beatriz G.

AU - Brito, Ivina R.

AU - Silva, Gerlane M.

AU - Rodrigues, Giovanna Q.

AU - Nunes-Pinheiro, Diana C.S.

AU - Sales, Antônia D.

AU - Moura, Arlindo A.

AU - Wheeler, Matthew B.

AU - Rodrigues, Ana Paula R.

AU - Campello, Cláudio C.

AU - Figueiredo, José Ricardo

PY - 2017/4/1

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AB - The aims of this study were to investigate the effects of medium replacement system (experiment I) and of FSH presentations (homeopathic – FSH 6cH and allopathic FSH – rFSH; experiment II) on the in vitro development, hormone production and gene expression of isolated ovine preantral follicles cultured for 6 days. In experiment I, secondary follicles were cultured in the α-MEM+ supplemented with FSH 6cH (0.05 fg/ml) or recombinant bovine FSH (100 ng/ml) without/with daily medium addition. The homeopathic FSH treatments with/without medium addition improved (p <.05) follicular development compared to rFSH100 treatment without addition. FSH 6cH with addition showed the highest (p <.05) estradiol production. To verify whether the effects of homeopathic FSH were not due to its vehicle, experiment II was performed. The α-MEM+ was supplemented or not with alcohol (0.2% grain ethanol, v/v), FSH 6cH or rFSH100 with daily medium addition. Surprisingly, we found that all treatments improved follicular development compared to the α-MEM+ (p <.05). Moreover, homeopathic FSH was similar to the other treatments including its vehicle. In conclusion, its vehicle (ethanol) causes the effect of homeopathic FSH on in vitro development of isolated ovine preantral follicles.

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KW - homeopathy

KW - hormone

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KW - ovarian follicles

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Unexpected effect of the vehicle (grain ethanol) of homeopathic FSH on the in vitro survival and development of isolated ovine preantral follicles

Abstract

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