Epigenetic modifications play an important role in regulating gene expression among a host of other factors during cell development. The plasticity of epigenetic modifications has the potential to direct a cell in relation to environmental factors to its destined state. Therefore, understanding different epigenetic states is crucial for dissecting the gene regulatory mechanisms. With the recent advances in single-molecule tools, monitoring the dynamics of epigenetic regulation and quantification has become possible. In this review, we discuss the recent advances in single-molecule fluorescence tools in gaining new insights into the epigenetic regulation and its relation to chromatin conformation.

Original languageEnglish (US)
Pages (from-to)18-24
Number of pages7
JournalCurrent Opinion in Biomedical Engineering
StatePublished - Dec 2019


  • Chromatin structure
  • Epigenetics
  • Fluorescence correlation spectroscopy (FCS)
  • Fluorescence recovery after photobleaching (FRAP)
  • Förster resonance energy transfer (FRET)
  • Super-resolution

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Bioengineering
  • Medicine (miscellaneous)


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