Abstract
The synaptic inputs and outputs of the major interneuron L10 of the abdominal ganglion of Aplysia were studied using an intracellular staining technique for the electron microscope. The sites of both the chemical synaptic input and output of L10 are localized to the dendritic arborizations that arise from the axon in the ganglion neuropil. Thus, the interneuronal functions are mediated at the dendritic processes and could occur in the absence of spiking in the axon and cell body. The sites of L10 synaptic output are presumed to be at. aggregations of vesicles and mitochondria in the dendrites. The synaptic vesicle content of L10, a cholinergic neuron, with many large dense vesicles resembles that described for serotonergic cells in Aplysia, making distinction of synaptic pharmacology by ultrastructure difficult. Focal membrane specializations with a clear synaptic cleft were not observed between L10 and its large population of postsynaptic cells. In contrast, clear focal input sites were frequently found on L10. Gap junctions, sites of probable electrical coupling between L10 and other neurons, were also found. These observations are discussed as evidence that many synapses do not have focal specializations.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 463-474 |
| Number of pages | 12 |
| Journal | Journal of Neurobiology |
| Volume | 6 |
| Issue number | 5 |
| DOIs | |
| State | Published - Sep 1975 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Neuroscience(all)
- Cellular and Molecular Neuroscience
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Ultrastructural correlates of interneuronal function in the abdominal ganglion of Aplysia californica. / Gillette, Rhanor; Pomeranz, Bruce.
In: Journal of Neurobiology, Vol. 6, No. 5, 09.1975, p. 463-474.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ultrastructural correlates of interneuronal function in the abdominal ganglion of Aplysia californica
AU - Gillette, Rhanor
AU - Pomeranz, Bruce
PY - 1975/9
Y1 - 1975/9
N2 - The synaptic inputs and outputs of the major interneuron L10 of the abdominal ganglion of Aplysia were studied using an intracellular staining technique for the electron microscope. The sites of both the chemical synaptic input and output of L10 are localized to the dendritic arborizations that arise from the axon in the ganglion neuropil. Thus, the interneuronal functions are mediated at the dendritic processes and could occur in the absence of spiking in the axon and cell body. The sites of L10 synaptic output are presumed to be at. aggregations of vesicles and mitochondria in the dendrites. The synaptic vesicle content of L10, a cholinergic neuron, with many large dense vesicles resembles that described for serotonergic cells in Aplysia, making distinction of synaptic pharmacology by ultrastructure difficult. Focal membrane specializations with a clear synaptic cleft were not observed between L10 and its large population of postsynaptic cells. In contrast, clear focal input sites were frequently found on L10. Gap junctions, sites of probable electrical coupling between L10 and other neurons, were also found. These observations are discussed as evidence that many synapses do not have focal specializations.
AB - The synaptic inputs and outputs of the major interneuron L10 of the abdominal ganglion of Aplysia were studied using an intracellular staining technique for the electron microscope. The sites of both the chemical synaptic input and output of L10 are localized to the dendritic arborizations that arise from the axon in the ganglion neuropil. Thus, the interneuronal functions are mediated at the dendritic processes and could occur in the absence of spiking in the axon and cell body. The sites of L10 synaptic output are presumed to be at. aggregations of vesicles and mitochondria in the dendrites. The synaptic vesicle content of L10, a cholinergic neuron, with many large dense vesicles resembles that described for serotonergic cells in Aplysia, making distinction of synaptic pharmacology by ultrastructure difficult. Focal membrane specializations with a clear synaptic cleft were not observed between L10 and its large population of postsynaptic cells. In contrast, clear focal input sites were frequently found on L10. Gap junctions, sites of probable electrical coupling between L10 and other neurons, were also found. These observations are discussed as evidence that many synapses do not have focal specializations.
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UR - http://www.scopus.com/inward/citedby.url?scp=0016774181&partnerID=8YFLogxK
U2 - 10.1002/neu.480060504
DO - 10.1002/neu.480060504
M3 - Article
C2 - 1176980
AN - SCOPUS:0016774181
VL - 6
SP - 463
EP - 474
JO - Developmental Neurobiology
JF - Developmental Neurobiology
SN - 1932-8451
IS - 5
ER -