Ultrastructural correlates of interneuronal function in the abdominal ganglion of Aplysia californica

Rhanor Gillette, Bruce Pomeranz

Research output: Contribution to journalArticle

Abstract

The synaptic inputs and outputs of the major interneuron L10 of the abdominal ganglion of Aplysia were studied using an intracellular staining technique for the electron microscope. The sites of both the chemical synaptic input and output of L10 are localized to the dendritic arborizations that arise from the axon in the ganglion neuropil. Thus, the interneuronal functions are mediated at the dendritic processes and could occur in the absence of spiking in the axon and cell body. The sites of L10 synaptic output are presumed to be at. aggregations of vesicles and mitochondria in the dendrites. The synaptic vesicle content of L10, a cholinergic neuron, with many large dense vesicles resembles that described for serotonergic cells in Aplysia, making distinction of synaptic pharmacology by ultrastructure difficult. Focal membrane specializations with a clear synaptic cleft were not observed between L10 and its large population of postsynaptic cells. In contrast, clear focal input sites were frequently found on L10. Gap junctions, sites of probable electrical coupling between L10 and other neurons, were also found. These observations are discussed as evidence that many synapses do not have focal specializations.

Original languageEnglish (US)
Pages (from-to)463-474
Number of pages12
JournalJournal of Neurobiology
Volume6
Issue number5
DOIs
StatePublished - Sep 1975
Externally publishedYes

Fingerprint

Aplysia
Ganglia
Axons
Neuronal Plasticity
Cholinergic Neurons
Neuropil
Synaptic Vesicles
Gap Junctions
Interneurons
Dendrites
Synapses
Mitochondria
Pharmacology
Electrons
Staining and Labeling
Neurons
Membranes
Population
Cell Body

ASJC Scopus subject areas

  • Neuroscience(all)
  • Cellular and Molecular Neuroscience

Cite this

Ultrastructural correlates of interneuronal function in the abdominal ganglion of Aplysia californica. / Gillette, Rhanor; Pomeranz, Bruce.

In: Journal of Neurobiology, Vol. 6, No. 5, 09.1975, p. 463-474.

Research output: Contribution to journalArticle

@article{0ab771d26a0d499baed0913a283f02d6,
title = "Ultrastructural correlates of interneuronal function in the abdominal ganglion of Aplysia californica",
abstract = "The synaptic inputs and outputs of the major interneuron L10 of the abdominal ganglion of Aplysia were studied using an intracellular staining technique for the electron microscope. The sites of both the chemical synaptic input and output of L10 are localized to the dendritic arborizations that arise from the axon in the ganglion neuropil. Thus, the interneuronal functions are mediated at the dendritic processes and could occur in the absence of spiking in the axon and cell body. The sites of L10 synaptic output are presumed to be at. aggregations of vesicles and mitochondria in the dendrites. The synaptic vesicle content of L10, a cholinergic neuron, with many large dense vesicles resembles that described for serotonergic cells in Aplysia, making distinction of synaptic pharmacology by ultrastructure difficult. Focal membrane specializations with a clear synaptic cleft were not observed between L10 and its large population of postsynaptic cells. In contrast, clear focal input sites were frequently found on L10. Gap junctions, sites of probable electrical coupling between L10 and other neurons, were also found. These observations are discussed as evidence that many synapses do not have focal specializations.",
author = "Rhanor Gillette and Bruce Pomeranz",
year = "1975",
month = "9",
doi = "10.1002/neu.480060504",
language = "English (US)",
volume = "6",
pages = "463--474",
journal = "Developmental Neurobiology",
issn = "1932-8451",
publisher = "John Wiley and Sons Inc.",
number = "5",

}

TY - JOUR

T1 - Ultrastructural correlates of interneuronal function in the abdominal ganglion of Aplysia californica

AU - Gillette, Rhanor

AU - Pomeranz, Bruce

PY - 1975/9

Y1 - 1975/9

N2 - The synaptic inputs and outputs of the major interneuron L10 of the abdominal ganglion of Aplysia were studied using an intracellular staining technique for the electron microscope. The sites of both the chemical synaptic input and output of L10 are localized to the dendritic arborizations that arise from the axon in the ganglion neuropil. Thus, the interneuronal functions are mediated at the dendritic processes and could occur in the absence of spiking in the axon and cell body. The sites of L10 synaptic output are presumed to be at. aggregations of vesicles and mitochondria in the dendrites. The synaptic vesicle content of L10, a cholinergic neuron, with many large dense vesicles resembles that described for serotonergic cells in Aplysia, making distinction of synaptic pharmacology by ultrastructure difficult. Focal membrane specializations with a clear synaptic cleft were not observed between L10 and its large population of postsynaptic cells. In contrast, clear focal input sites were frequently found on L10. Gap junctions, sites of probable electrical coupling between L10 and other neurons, were also found. These observations are discussed as evidence that many synapses do not have focal specializations.

AB - The synaptic inputs and outputs of the major interneuron L10 of the abdominal ganglion of Aplysia were studied using an intracellular staining technique for the electron microscope. The sites of both the chemical synaptic input and output of L10 are localized to the dendritic arborizations that arise from the axon in the ganglion neuropil. Thus, the interneuronal functions are mediated at the dendritic processes and could occur in the absence of spiking in the axon and cell body. The sites of L10 synaptic output are presumed to be at. aggregations of vesicles and mitochondria in the dendrites. The synaptic vesicle content of L10, a cholinergic neuron, with many large dense vesicles resembles that described for serotonergic cells in Aplysia, making distinction of synaptic pharmacology by ultrastructure difficult. Focal membrane specializations with a clear synaptic cleft were not observed between L10 and its large population of postsynaptic cells. In contrast, clear focal input sites were frequently found on L10. Gap junctions, sites of probable electrical coupling between L10 and other neurons, were also found. These observations are discussed as evidence that many synapses do not have focal specializations.

UR - http://www.scopus.com/inward/record.url?scp=0016774181&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016774181&partnerID=8YFLogxK

U2 - 10.1002/neu.480060504

DO - 10.1002/neu.480060504

M3 - Article

C2 - 1176980

AN - SCOPUS:0016774181

VL - 6

SP - 463

EP - 474

JO - Developmental Neurobiology

JF - Developmental Neurobiology

SN - 1932-8451

IS - 5

ER -