TY - JOUR
T1 - Ultrasound Scattering From Cell-Pellet Biophantoms and Ex Vivo Tumors Provides Insight Into the Cellular Structure Involved in Scattering
AU - Muleki-Seya, Pauline
AU - O'Brien, William D.
N1 - Publisher Copyright:
1525-8955 © 2021 IEEE. Personal use is permitted, but republication/redistribution requires IEEE permission. See https://www.ieee.org/publications/rights/index.html for more information.
PY - 2022/2/1
Y1 - 2022/2/1
N2 - The histologically identifiable cellular structure(s) involved in ultrasonic scattering is(are) yet to be uniquely identified. The study quantifies six possible cellular scattering parameters, namely, cell and nucleus radii and their respective cell and nucleus volume fractions as well as a combination of cell and nucleus radii and their volume fraction. The six cellular parameters are each derived from four cell lines (4T1, JC, LMTK, and MAT) and two tissue types (cell-pellet biophantom and ex vivo tumor). Optical histology and quantitative ultrasound (QUS), both independent approaches, are used to yield these cellular parameters. QUS scatterer parameters are experimentally determined using two ultrasonic scattering models: the spherical Gaussian model (GM) and the structure factor model (SFM) to yield insight about scattering from nuclei only and cells only. GM is a classical ultrasonic scattering model to evaluate QUS parameters and is well adapted for diluted media. SFM is adapted for dense media to estimate reasonably well scatterer parameters of cellular structures from ex vivo tissue. Nucleus and cell radii and volume fractions are measured optically from histology. They were used as inputs to calculate BSC for scattering from cells, nuclei, and both cells and nuclei. The QUS-derived scatterers (radii and volume fractions) distributions were then compared to the optical histology scatterer parameters derived from these calculated BSCs. The results suggest scattering from cells only (LMTK and MAT) or both cells and nuclei (4T1 and JC) for cell-pellet biophantoms and scattering from nuclei only for tumors.
AB - The histologically identifiable cellular structure(s) involved in ultrasonic scattering is(are) yet to be uniquely identified. The study quantifies six possible cellular scattering parameters, namely, cell and nucleus radii and their respective cell and nucleus volume fractions as well as a combination of cell and nucleus radii and their volume fraction. The six cellular parameters are each derived from four cell lines (4T1, JC, LMTK, and MAT) and two tissue types (cell-pellet biophantom and ex vivo tumor). Optical histology and quantitative ultrasound (QUS), both independent approaches, are used to yield these cellular parameters. QUS scatterer parameters are experimentally determined using two ultrasonic scattering models: the spherical Gaussian model (GM) and the structure factor model (SFM) to yield insight about scattering from nuclei only and cells only. GM is a classical ultrasonic scattering model to evaluate QUS parameters and is well adapted for diluted media. SFM is adapted for dense media to estimate reasonably well scatterer parameters of cellular structures from ex vivo tissue. Nucleus and cell radii and volume fractions are measured optically from histology. They were used as inputs to calculate BSC for scattering from cells, nuclei, and both cells and nuclei. The QUS-derived scatterers (radii and volume fractions) distributions were then compared to the optical histology scatterer parameters derived from these calculated BSCs. The results suggest scattering from cells only (LMTK and MAT) or both cells and nuclei (4T1 and JC) for cell-pellet biophantoms and scattering from nuclei only for tumors.
KW - Acoustics
KW - Biomedical optical imaging
KW - Histopathology
KW - Optical imaging
KW - Optical scattering
KW - Scattering
KW - Tumors
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U2 - 10.1109/TUFFC.2021.3130682
DO - 10.1109/TUFFC.2021.3130682
M3 - Article
C2 - 34822328
AN - SCOPUS:85120539353
SN - 0885-3010
VL - 69
SP - 637
EP - 649
JO - IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
JF - IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
IS - 2
ER -