Ultrasensitive protein detection in blood serum using gold nanoparticle probes by single molecule spectroscopy

Jiji Chen, Chungang Wang, Joseph Irudayaraj

Research output: Contribution to journalArticle

Abstract

A one-step rapid and ultrasensitive immunoassay capable of detecting proteins in blood serum is developed using gold nanoprobes and fluorescence correlation spectroscopy (FCS). In this approach we take advantage of the inherent photoluminescence property of gold nanoparticles (GNPs) to develop a fluorophore-free assay to observe binding entities by monitoring the diffusion of bound versus unbound molecules in a limited confocal volume. 40-nm GNPs conjugated separately with rabbit anti-IgG (Fc) and goat anti-IgG (Fab) when incubated in blood serum containing IgG forms a sandwich structure constituting dimers and oligomers that can be differentiated by to detect IgG in blood serum at a limit of detection (LOD) of 5pg/ml. The novelty of integrating GNPs with FCS to develop a sensitive blood immunoassay brings single molecule methods one step closer to the clinic.

Original languageEnglish (US)
Article number040501
JournalJournal of biomedical optics
Volume14
Issue number4
DOIs
StatePublished - Dec 1 2009

Fingerprint

blood serum
Gold
Blood
Spectroscopy
gold
Nanoparticles
proteins
Proteins
nanoparticles
immunoassay
Molecules
probes
spectroscopy
molecules
Immunoglobulin G
Fluorescence
goats
Nanoprobes
fluorescence
Sandwich structures

Keywords

  • blood serum
  • detection
  • fluorescence correlation spectroscopy
  • gold nanoparticles

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

Ultrasensitive protein detection in blood serum using gold nanoparticle probes by single molecule spectroscopy. / Chen, Jiji; Wang, Chungang; Irudayaraj, Joseph.

In: Journal of biomedical optics, Vol. 14, No. 4, 040501, 01.12.2009.

Research output: Contribution to journalArticle

@article{a6834b892f4d463ab2f99d32810d9dd8,
title = "Ultrasensitive protein detection in blood serum using gold nanoparticle probes by single molecule spectroscopy",
abstract = "A one-step rapid and ultrasensitive immunoassay capable of detecting proteins in blood serum is developed using gold nanoprobes and fluorescence correlation spectroscopy (FCS). In this approach we take advantage of the inherent photoluminescence property of gold nanoparticles (GNPs) to develop a fluorophore-free assay to observe binding entities by monitoring the diffusion of bound versus unbound molecules in a limited confocal volume. 40-nm GNPs conjugated separately with rabbit anti-IgG (Fc) and goat anti-IgG (Fab) when incubated in blood serum containing IgG forms a sandwich structure constituting dimers and oligomers that can be differentiated by to detect IgG in blood serum at a limit of detection (LOD) of 5pg/ml. The novelty of integrating GNPs with FCS to develop a sensitive blood immunoassay brings single molecule methods one step closer to the clinic.",
keywords = "blood serum, detection, fluorescence correlation spectroscopy, gold nanoparticles",
author = "Jiji Chen and Chungang Wang and Joseph Irudayaraj",
year = "2009",
month = "12",
day = "1",
doi = "10.1117/1.3183789",
language = "English (US)",
volume = "14",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
publisher = "SPIE",
number = "4",

}

TY - JOUR

T1 - Ultrasensitive protein detection in blood serum using gold nanoparticle probes by single molecule spectroscopy

AU - Chen, Jiji

AU - Wang, Chungang

AU - Irudayaraj, Joseph

PY - 2009/12/1

Y1 - 2009/12/1

N2 - A one-step rapid and ultrasensitive immunoassay capable of detecting proteins in blood serum is developed using gold nanoprobes and fluorescence correlation spectroscopy (FCS). In this approach we take advantage of the inherent photoluminescence property of gold nanoparticles (GNPs) to develop a fluorophore-free assay to observe binding entities by monitoring the diffusion of bound versus unbound molecules in a limited confocal volume. 40-nm GNPs conjugated separately with rabbit anti-IgG (Fc) and goat anti-IgG (Fab) when incubated in blood serum containing IgG forms a sandwich structure constituting dimers and oligomers that can be differentiated by to detect IgG in blood serum at a limit of detection (LOD) of 5pg/ml. The novelty of integrating GNPs with FCS to develop a sensitive blood immunoassay brings single molecule methods one step closer to the clinic.

AB - A one-step rapid and ultrasensitive immunoassay capable of detecting proteins in blood serum is developed using gold nanoprobes and fluorescence correlation spectroscopy (FCS). In this approach we take advantage of the inherent photoluminescence property of gold nanoparticles (GNPs) to develop a fluorophore-free assay to observe binding entities by monitoring the diffusion of bound versus unbound molecules in a limited confocal volume. 40-nm GNPs conjugated separately with rabbit anti-IgG (Fc) and goat anti-IgG (Fab) when incubated in blood serum containing IgG forms a sandwich structure constituting dimers and oligomers that can be differentiated by to detect IgG in blood serum at a limit of detection (LOD) of 5pg/ml. The novelty of integrating GNPs with FCS to develop a sensitive blood immunoassay brings single molecule methods one step closer to the clinic.

KW - blood serum

KW - detection

KW - fluorescence correlation spectroscopy

KW - gold nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=73349083422&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=73349083422&partnerID=8YFLogxK

U2 - 10.1117/1.3183789

DO - 10.1117/1.3183789

M3 - Article

C2 - 19725706

AN - SCOPUS:73349083422

VL - 14

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 4

M1 - 040501

ER -