TY - JOUR
T1 - Ultrasensitive detection of nucleic acids using deformed graphene channel field effect biosensors
AU - Hwang, Michael Taeyoung
AU - Heiranian, Mohammad
AU - Kim, Yerim
AU - You, Seungyong
AU - Leem, Juyoung
AU - Taqieddin, Amir
AU - Faramarzi, Vahid
AU - Jing, Yuhang
AU - Park, Insu
AU - van der Zande, Arend M.
AU - Nam, Sungwoo
AU - Aluru, Narayana R.
AU - Bashir, Rashid
N1 - This research was primarily supported by the NSF through the University of Illinois at Urbana-Champaign Materials Research Science and Engineering Center DMR-1720633. The work was carried out in part in the Holonyak Micro and Nanotechnology Laboratory and the Material Research Laboratory Central Facilities at University of Illinois. We are grateful to our colleagues, Paolo Ferrari, Jordan Dennison, Minji Chang and Ashley Walker for help in the experimentation process as well as insights during the course of this research. We also appreciate Scott K. Silverman and Chih-Cheng Yeh for planning and performing the radioactive labeling quantification. The simulations were performed using the Extreme Science and Engineering Discovery Environment (XSEDE) (supported by National Science Foundation (NSF) Grant No. OCI1053575) and Blue Waters (supported by NSF awards OCI-0725070, ACI-1238993 and the state of Illinois, and as of December, 2019, supported by the National Geospatial-Intelligence Agency).
PY - 2020/3/24
Y1 - 2020/3/24
N2 - Field-effect transistor (FET)-based biosensors allow label-free detection of biomolecules by measuring their intrinsic charges. The detection limit of these sensors is determined by the Debye screening of the charges from counter ions in solutions. Here, we use FETs with a deformed monolayer graphene channel for the detection of nucleic acids. These devices with even millimeter scale channels show an ultra-high sensitivity detection in buffer and human serum sample down to 600 zM and 20 aM, respectively, which are ∼18 and ∼600 nucleic acid molecules. Computational simulations reveal that the nanoscale deformations can form ‘electrical hot spots’ in the sensing channel which reduce the charge screening at the concave regions. Moreover, the deformed graphene could exhibit a band-gap, allowing an exponential change in the source-drain current from small numbers of charges. Collectively, these phenomena allow for ultrasensitive electronic biomolecular detection in millimeter scale structures.
AB - Field-effect transistor (FET)-based biosensors allow label-free detection of biomolecules by measuring their intrinsic charges. The detection limit of these sensors is determined by the Debye screening of the charges from counter ions in solutions. Here, we use FETs with a deformed monolayer graphene channel for the detection of nucleic acids. These devices with even millimeter scale channels show an ultra-high sensitivity detection in buffer and human serum sample down to 600 zM and 20 aM, respectively, which are ∼18 and ∼600 nucleic acid molecules. Computational simulations reveal that the nanoscale deformations can form ‘electrical hot spots’ in the sensing channel which reduce the charge screening at the concave regions. Moreover, the deformed graphene could exhibit a band-gap, allowing an exponential change in the source-drain current from small numbers of charges. Collectively, these phenomena allow for ultrasensitive electronic biomolecular detection in millimeter scale structures.
KW - biomedical engineering
KW - biosensors
KW - materials science
KW - mechanical and structural properties and devices
UR - https://www.scopus.com/pages/publications/85082380883
UR - https://www.scopus.com/pages/publications/85082380883#tab=citedBy
U2 - 10.1038/s41467-020-15330-9
DO - 10.1038/s41467-020-15330-9
M3 - Article
C2 - 32210235
SN - 2041-1723
VL - 11
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 1543
ER -