Ultralocalized thermal reactions in subnanoliter droplets-in-air

Eric Salm, Carlos Duarte Guevara, Piyush Dak, Brian Ross Dorvel, Bobby Reddy, Muhammad Ashraf Alam, Rashid Bashir

Research output: Contribution to journalArticle

Abstract

Miniaturized laboratory-on-chip systems promise rapid, sensitive, and multiplexed detection of biological samples for medical diagnostics, drug discovery, and high-throughput screening. Within miniaturized laboratory-on-chips, static and dynamic droplets of fluids in different immiscible media have been used as individual vessels to perform biochemical reactions and confine the products. Approaches to performlocalized heating of these individual subnanoliter droplets can allow for new applications that require parallel, time-, and spacemultiplex reactions on a single integrated circuit. Our method positions droplets on an array of individual silicon microwave heaters on chip to precisely control the temperature of droplets-in-air, allowing us to perform biochemical reactions, including DNA melting and detection of single base mismatches. We also demonstrate that ssDNA probemolecules can be placed on heaters in solution, dried, and then rehydrated by ssDNA target molecules in droplets for hybridization and detection. This platform enables many applications in droplets including hybridization of lowcopy number DNAmolecules, lysing of single cells, interrogation of ligand-receptor interactions, and rapid temperature cycling for amplification of DNA molecules.

Original languageEnglish (US)
Pages (from-to)3310-3315
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number9
DOIs
StatePublished - Feb 26 2013

Keywords

  • Evaporation
  • Nanowire
  • On-chip heating

ASJC Scopus subject areas

  • General

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