@article{93d5f2975c964c6b8993afab828020d0,
title = "Ultra-parallel label-free optophysiology of neural activity",
abstract = "The electrical activity of neurons has a spatiotemporal footprint that spans three orders of magnitude. Traditional electrophysiology lacks the spatial throughput to image the activity of an entire neural network; besides, labeled optical imaging using voltage-sensitive dyes and tracking Ca2+ ion dynamics lack the versatility and speed to capture fast-spiking activity, respectively. We present a label-free optical imaging technique to image the changes to the optical path length and the local birefringence caused by neural activity, at 4,000 Hz, across a 200 × 200 μm2 region, and with micron-scale spatial resolution and 300-pm displacement sensitivity using Superfast Polarization-sensitive Off-axis Full-field Optical Coherence Microscopy (SPoOF OCM). The undulations in the optical responses from mammalian neuronal activity were matched with field-potential electrophysiology measurements and validated with channel blockers. By directly tracking the widefield neural activity at millisecond timescales and micrometer resolution, SPoOF OCM provides a framework to progress from low-throughput electrophysiology to high-throughput ultra-parallel label-free optophysiology.",
keywords = "Cell biology, Neuroscience, Optical imaging",
author = "Iyer, {Rishyashring R.} and Liu, {Yuan Zhi} and Renteria, {Carlos A.} and Tibble, {Brian E.} and Honggu Choi and Mantas {\v Z}urauskas and Boppart, {Stephen A.}",
note = "The authors would like to acknowledge Eric Chaney and Dr. Edita Aksamitiene for their assistance with cell cultures, Dr. Jianfeng Wang and Darold R. Spillman Jr. for their assistance with system setup, and Janet E. Sorrells and Lingxiao Yang for their critique on the figures and visualizations. Additional information can be found at: biophotonics.illinois.edu . This research was supported in part by grants from the National Institutes of Health ( R01CA213149, R01EB023232, and R01CA241618 ) and the Air Force Office of Scientific Research ( FA9550-17-1-0387 ). C.A.R. was supported by an NIH / NIEHS Fellowship Training Program in Endocrine, Developmental and Reproductive Toxicology ( T32ES007326 ). R.R.I. was supported in part by the Mavis Future Faculty Fellows program from the Grainger College of Engineering at the University of Illinois at Urbana-Champaign and supported in part by the Tissue Microenvironment Training Program funded by the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health ( T32EB019944 ). The authors would like to acknowledge Eric Chaney and Dr. Edita Aksamitiene for their assistance with cell cultures, Dr. Jianfeng Wang and Darold R. Spillman Jr. for their assistance with system setup, and Janet E. Sorrells and Lingxiao Yang for their critique on the figures and visualizations. Additional information can be found at: biophotonics.illinois.edu. This research was supported in part by grants from the National Institutes of Health (R01CA213149, R01EB023232, and R01CA241618) and the Air Force Office of Scientific Research (FA9550-17-1-0387). C.A.R. was supported by an NIH/NIEHS Fellowship Training Program in Endocrine, Developmental and Reproductive Toxicology (T32ES007326). R.R.I. was supported in part by the Mavis Future Faculty Fellows program from the Grainger College of Engineering at the University of Illinois at Urbana-Champaign and supported in part by the Tissue Microenvironment Training Program funded by the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health (T32EB019944). Conceptualization, Y-Z.L. R.R.I. and S.A.B.; Software, Formal analysis, Visualization, and Investigation, R.R.I.; Resources: R.R.I. Y-Z.L. C.A.R. and B.E.T.; Methodology, R.R.I. C.A.R. H.C. and M.Z.; Writing, R.R.I. Y-Z.L. and S.A.B.; Supervision and Funding Acquisition, S.A.B. The authors declare no competing interests.",
year = "2022",
month = may,
day = "20",
doi = "10.1016/j.isci.2022.104307",
language = "English (US)",
volume = "25",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier Inc.",
number = "5",
}