Ultra-long DNA molecule isolation from plant nuclei for ultra-long read genome sequencing

Dessireé Zerpa-Catanho, Xiaodan Zhang, Jinjin Song, Alvaro G. Hernandez, Ray Ming

Research output: Contribution to journalArticlepeer-review

Abstract

Long and ultra-long read DNA sequencing technologies require high molecular weight DNA with high quality and sufficient quantity, which could be challenging to obtain from recalcitrant plant tissues. We describe a protocol to isolate ultra-long DNA from 12 species for ultra-long read genome sequencing. A suitable nuclei lysis buffer is critical for DNA quality and yield. This protocol will enable individual labs to isolate high molecular weight DNA at a rapid pace with low cost from a variety of plant species. For complete information on the use and execution of this protocol, please refer to: Zhang et al. (2020).

Original languageEnglish (US)
Article number100343
JournalSTAR Protocols
Volume2
Issue number1
DOIs
StatePublished - Mar 19 2021

Keywords

  • Genomics
  • Molecular biology
  • Sequencing

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience
  • General Immunology and Microbiology
  • General Medicine

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