Abstract
We report a label-free, highly sensitive biosensor using a vertical cavity surface emitting laser (VCSEL) based measurement system for the detection and monitoring of biomolecular interactions. The sensor system consists of a VCSEL, a plastic guided mode resonant (GMR) filter, and two pin detectors. The system has several advantages such as extreme compactness, high sensitivity, high throughput, fest measurements, low power consumption, low cost, and the potential to become portable. Experimentally, the biosensor system has shown to be highly sensitive to the surface modifications due to molecular bindings, with the ability to detect the thickness variations <10Å and retractive index variations <0.005. The biosensor also has demonstrated its high sensitivity for the detection of antibody-antigen proteins bindings, with the mouse IgG concentration as low as lpg/ml (6.7 femto-Molar), and its ability for measuring both static and dynamic interactions among proteins.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 140-146 |
| Number of pages | 7 |
| Journal | Proceedings of SPIE - The International Society for Optical Engineering |
| Volume | 5328 |
| DOIs | |
| State | Published - 2004 |
| Externally published | Yes |
| Event | Microarrays and Combinatorial Techniques: Design, Fabrication, and Analysis II - San Jose, CA, United States Duration: Jan 25 2004 → Jan 26 2004 |
Keywords
- Biomedical Transducer
- Drugs
- Dynamic Response
- Label-free
- Non-intrusive
- Protein
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering