Tyramide signal amplification mass spectrometry (TSA-MS) ratio identifies nuclear speckle proteins

Joseph Dopie, Michael J. Sweredoski, Annie Moradian, Andrew S. Belmont

Research output: Contribution to journalArticlepeer-review

Abstract

We present a simple ratio method to infer protein composition within cellular structures using proximity labeling approaches but compensating for the diffusion of free radicals. We used tyramide signal amplification (TSA) and label-free mass spectrometry (MS) to compare proteins in nuclear speckles versus centromeres. Our TSA-MS ratio approach successfully identified known nuclear speckle proteins. For example, 96% and 67% of proteins in the top 30 and 100 sorted proteins, respectively, are known nuclear speckle proteins, including proteins that we validated here as enriched in nuclear speckles. We show that MFAP1, among the top 20 in our list, forms droplets under certain circumstances and that MFAP1 expression levels modulate the size, stability, and dynamics of nuclear speckles. Localization of MFAP1 and its binding partner, PRPF38A, in droplet-like nuclear bodies precedes formation of nuclear speckles during telophase. Our results update older proteomic studies of nuclear speckles and should provide a useful reference dataset to guide future experimental dissection of nuclear speckle structure and function.

Original languageEnglish (US)
Article numberjcb.201910207
JournalJournal of Cell Biology
Volume219
Issue number9
DOIs
StatePublished - Sep 7 2020

ASJC Scopus subject areas

  • Cell Biology

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