TY - JOUR
T1 - Type VI secretion systems of Erwinia amylovora contribute to bacterial competition, virulence, and exopolysaccharide production
AU - Tian, Yanli
AU - Zhao, Yuqiang
AU - Shi, Linye
AU - Cui, Zhongli
AU - Hu, Baishi
AU - Zhao, Youfu
N1 - Publisher Copyright:
© 2017 The American Phytopathological Society.
PY - 2017/6
Y1 - 2017/6
N2 - The type VI secretion system (T6SS) plays a major role in mediating interbacterial competition and might contribute to virulence in plant pathogenic bacteria. However, the role of T6SS in Erwinia amylovora remains unknown. In this study, 33 deletion mutants within three T6SS clusters were generated in E. amylovora strain NCPPB1665. Our results showed that all 33 mutants displayed reduced antibacterial activities against Escherichia coli as compared with that of the wild-type (WT) strain, indicating that Erwinia amylovora T6SS are functional. Of the 33 mutants, 19 exhibited reduced virulence on immature pear fruit as compared with that of the WT strain. Among them, 6, 1, and 12 genes belonged to T6SS-1, T6SS-2, and T6SS-3 clusters, respectively. Interestingly, these 19 mutants also produced less amylovoran or levan or both. These findings suggest that E. amylovora T6SS play a role in bacterial competition and virulence possibly by influencing exopolysaccharide production.
AB - The type VI secretion system (T6SS) plays a major role in mediating interbacterial competition and might contribute to virulence in plant pathogenic bacteria. However, the role of T6SS in Erwinia amylovora remains unknown. In this study, 33 deletion mutants within three T6SS clusters were generated in E. amylovora strain NCPPB1665. Our results showed that all 33 mutants displayed reduced antibacterial activities against Escherichia coli as compared with that of the wild-type (WT) strain, indicating that Erwinia amylovora T6SS are functional. Of the 33 mutants, 19 exhibited reduced virulence on immature pear fruit as compared with that of the WT strain. Among them, 6, 1, and 12 genes belonged to T6SS-1, T6SS-2, and T6SS-3 clusters, respectively. Interestingly, these 19 mutants also produced less amylovoran or levan or both. These findings suggest that E. amylovora T6SS play a role in bacterial competition and virulence possibly by influencing exopolysaccharide production.
KW - Protein secretion system
UR - http://www.scopus.com/inward/record.url?scp=85020427013&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85020427013&partnerID=8YFLogxK
U2 - 10.1094/PHYTO-11-16-0393-R
DO - 10.1094/PHYTO-11-16-0393-R
M3 - Article
C2 - 28421913
AN - SCOPUS:85020427013
SN - 0031-949X
VL - 107
SP - 654
EP - 661
JO - Phytopathology
JF - Phytopathology
IS - 6
ER -