TY - JOUR
T1 - Two stage cadherin kinetics require multiple extracellular domains but not the cytoplasmic region
AU - Chien, Yuan Hung
AU - Jiang, Ning
AU - Li, Fang
AU - Zhang, Fang
AU - Zhu, Cheng
AU - Leckband, Deborah
PY - 2008/1/25
Y1 - 2008/1/25
N2 - Micropipette manipulation measurements quantified the pre-steady state binding kinetics between cell pairs mediated by Xenopus cleavage stage cadherin. The time-dependence of the intercellular binding probability exhibits a fast forming, low probability binding state, which transitions to a slower forming, high probability state. The biphasic kinetics are independent of the cytoplasmic region, but the transition to the high probability state requires the third extracellular domain EC3. Deleting either EC3 or EC3-5, or substituting Trp 2 for Ala reduces the binding curves to a simple, monophasic rise in binding probability to a limiting plateau, as predicted for a single site binding mechanism. The two stage cadherin binding process reported here directly parallels previous biophysical studies, and confirms that the cadherin ectodomain governs the initial intercellular adhesion dynamics.
AB - Micropipette manipulation measurements quantified the pre-steady state binding kinetics between cell pairs mediated by Xenopus cleavage stage cadherin. The time-dependence of the intercellular binding probability exhibits a fast forming, low probability binding state, which transitions to a slower forming, high probability state. The biphasic kinetics are independent of the cytoplasmic region, but the transition to the high probability state requires the third extracellular domain EC3. Deleting either EC3 or EC3-5, or substituting Trp 2 for Ala reduces the binding curves to a simple, monophasic rise in binding probability to a limiting plateau, as predicted for a single site binding mechanism. The two stage cadherin binding process reported here directly parallels previous biophysical studies, and confirms that the cadherin ectodomain governs the initial intercellular adhesion dynamics.
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U2 - 10.1074/jbc.M708044200
DO - 10.1074/jbc.M708044200
M3 - Article
C2 - 17999960
AN - SCOPUS:38349096437
SN - 0021-9258
VL - 283
SP - 1848
EP - 1856
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 4
ER -