Two-stage acidic-alkaline hydrothermal pretreatment of lignocellulose for the high recovery of cellulose and hemicellulose sugars

Bin Guo, Yuanhui Zhang, Guo Yu, Won Heong Lee, Yong Su Jin, Eberhard Morgenroth

Research output: Contribution to journalArticlepeer-review

Abstract

The focus of this work was to develop a combined acid and alkaline hydrothermal pretreatment of lignocellulose that ensures high recovery of both hexose and pentose. Dilute sulfuric acid and lime pretreatments were employed sequentially. Process performance was optimized in terms of catalyst concentration, retention time, and temperature using response surface methodology. Medium operational conditions in the acid stage and harsh conditions in the alkaline stage were desirable with optimal performance at 0.73 wt% H2SO4, 150 °C, 6.1 min in the first stage, and 0.024 g lime/g biomass, 202 °C, 30 min in the second stage. In comparison to single-stage pretreatments with high recovery of either glucose or xylose, two-stage process showed great promises with >80 % glucose and >70 % xylose recovery. In addition, the method greatly improved ethanol fermentation with yields up to 0.145 g/g Miscanthus, due to significantly reduced formation of inhibitory by-products such as weak acids, furans, and phenols. Supplementing biomimetic acids would further increase glucose yield by up to 15 % and xylose yield by 25 %.

Original languageEnglish (US)
Pages (from-to)1069-1087
Number of pages19
JournalApplied Biochemistry and Biotechnology
Volume169
Issue number4
DOIs
StatePublished - Feb 2013

Keywords

  • Combined acid hydrolysis
  • Lignocellulose
  • Miscanthus
  • Response surface methodology
  • Two-stage acidic-alkaline pretreatment

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Environmental Engineering

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