Two-photon 3D fiona of individual quantum dots in an aqueous environment

Ruobing Zhang; Eli Rothenberg; Gilbert Fruhwirth; Paul D. Simonson; Fangfu Ye; Ido Golding; Tony Ng; Ward Lopes; Paul R Selvin

doi:10.1021/nl201225r

Two-photon 3D fiona of individual quantum dots in an aqueous environment

Ruobing Zhang, Eli Rothenberg, Gilbert Fruhwirth, Paul D. Simonson, Fangfu Ye, Ido Golding, Tony Ng, Ward Lopes, Paul R Selvin

Research output: Contribution to journal › Article › peer-review

Abstract

We report the first two-photon (2P) microscopy of individual quantum dots (QDs) in an aqueous environment with both widefield and point-scan excitations at nanometer accuracy. Thiol-containing reductants suppress QD blinking and enable measurement of the 36 nm step size of individual Myosin V motors in vitro. We localize QDs with an accuracy of 2-3 nm in all three dimensions by using a 9×9 matrix excitation hologram and an array detector, which also increases the 3D scan imaging rate by 80-fold. With this 3D microscopy we validate the LamB receptor distribution on E. coli and the endocytosis of EGF-receptors in breast cancer cells.

Original language	English (US)
Pages (from-to)	4074-4078
Number of pages	5
Journal	Nano letters
Volume	11
Issue number	10
DOIs	https://doi.org/10.1021/nl201225r
State	Published - Oct 12 2011

Keywords

EGFR
FIONA
Two photon
breast cancer
holographic excitation
quantum dot

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

Online availability

10.1021/nl201225r

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abstract = "We report the first two-photon (2P) microscopy of individual quantum dots (QDs) in an aqueous environment with both widefield and point-scan excitations at nanometer accuracy. Thiol-containing reductants suppress QD blinking and enable measurement of the 36 nm step size of individual Myosin V motors in vitro. We localize QDs with an accuracy of 2-3 nm in all three dimensions by using a 9×9 matrix excitation hologram and an array detector, which also increases the 3D scan imaging rate by 80-fold. With this 3D microscopy we validate the LamB receptor distribution on E. coli and the endocytosis of EGF-receptors in breast cancer cells.",

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AU - Zhang, Ruobing

AU - Rothenberg, Eli

AU - Fruhwirth, Gilbert

AU - Simonson, Paul D.

AU - Ye, Fangfu

AU - Golding, Ido

AU - Ng, Tony

AU - Lopes, Ward

AU - Selvin, Paul R

PY - 2011/10/12

Y1 - 2011/10/12

N2 - We report the first two-photon (2P) microscopy of individual quantum dots (QDs) in an aqueous environment with both widefield and point-scan excitations at nanometer accuracy. Thiol-containing reductants suppress QD blinking and enable measurement of the 36 nm step size of individual Myosin V motors in vitro. We localize QDs with an accuracy of 2-3 nm in all three dimensions by using a 9×9 matrix excitation hologram and an array detector, which also increases the 3D scan imaging rate by 80-fold. With this 3D microscopy we validate the LamB receptor distribution on E. coli and the endocytosis of EGF-receptors in breast cancer cells.

AB - We report the first two-photon (2P) microscopy of individual quantum dots (QDs) in an aqueous environment with both widefield and point-scan excitations at nanometer accuracy. Thiol-containing reductants suppress QD blinking and enable measurement of the 36 nm step size of individual Myosin V motors in vitro. We localize QDs with an accuracy of 2-3 nm in all three dimensions by using a 9×9 matrix excitation hologram and an array detector, which also increases the 3D scan imaging rate by 80-fold. With this 3D microscopy we validate the LamB receptor distribution on E. coli and the endocytosis of EGF-receptors in breast cancer cells.

KW - EGFR

KW - FIONA

KW - Two photon

KW - breast cancer

KW - holographic excitation

KW - quantum dot

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Two-photon 3D fiona of individual quantum dots in an aqueous environment

Abstract

Keywords

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