Two-photon 3D fiona of individual quantum dots in an aqueous environment

Ruobing Zhang, Eli Rothenberg, Gilbert Fruhwirth, Paul D. Simonson, Fangfu Ye, Ido Golding, Tony Ng, Ward Lopes, Paul R Selvin

Research output: Contribution to journalArticle

Abstract

We report the first two-photon (2P) microscopy of individual quantum dots (QDs) in an aqueous environment with both widefield and point-scan excitations at nanometer accuracy. Thiol-containing reductants suppress QD blinking and enable measurement of the 36 nm step size of individual Myosin V motors in vitro. We localize QDs with an accuracy of 2-3 nm in all three dimensions by using a 9×9 matrix excitation hologram and an array detector, which also increases the 3D scan imaging rate by 80-fold. With this 3D microscopy we validate the LamB receptor distribution on E. coli and the endocytosis of EGF-receptors in breast cancer cells.

Original languageEnglish (US)
Pages (from-to)4074-4078
Number of pages5
JournalNano letters
Volume11
Issue number10
DOIs
StatePublished - Oct 12 2011

Keywords

  • EGFR
  • FIONA
  • Two photon
  • breast cancer
  • holographic excitation
  • quantum dot

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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  • Cite this

    Zhang, R., Rothenberg, E., Fruhwirth, G., Simonson, P. D., Ye, F., Golding, I., Ng, T., Lopes, W., & Selvin, P. R. (2011). Two-photon 3D fiona of individual quantum dots in an aqueous environment. Nano letters, 11(10), 4074-4078. https://doi.org/10.1021/nl201225r