Two-dimensional pulsed electron spin resonance characterization of 15N-labeled archaeal Rieske-type ferredoxin

Toshio Iwasaki, Rimma I. Samoilova, Asako Kounosu, Sergei A. Dikanov

Research output: Contribution to journalArticlepeer-review

Abstract

Two-dimensional electron spin-echo envelope modulation (ESEEM) analysis of the uniformly 15N-labeled archaeal Rieske-type [2Fe-2S] ferredoxin (ARF) from Sulfolobus solfataricus P1 has been conducted in comparison with the previously characterized high-potential protein homologs. Major differences among these proteins were found in the hyperfine sublevel correlation (HYSCORE) lineshapes and intensities of the signals in the (++) quadrant, which are contributed from weakly coupled (non-coordinated) peptide nitrogens near the reduced clusters. They are less pronounced in the HYSCORE spectra of ARF than those of the high-potential protein homologs, and may account for the tuning of Rieske-type clusters in various redox systems.

Original languageEnglish (US)
Pages (from-to)3467-3472
Number of pages6
JournalFEBS Letters
Volume583
Issue number21
DOIs
StatePublished - Nov 3 2009

Keywords

  • Archaea
  • Electron paramagnetic resonance
  • Electron spin-echo envelope modulation
  • Ferredoxin
  • Hyperfine sublevel correlation
  • Rieske
  • [2Fe-2S] cluster

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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