Two-dimensional pulsed electron spin resonance characterization of 15N-labeled archaeal Rieske-type ferredoxin

Toshio Iwasaki; Rimma I. Samoilova; Asako Kounosu; Sergei A. Dikanov

doi:10.1016/j.febslet.2009.09.050

Two-dimensional pulsed electron spin resonance characterization of ¹⁵N-labeled archaeal Rieske-type ferredoxin

Toshio Iwasaki, Rimma I. Samoilova, Asako Kounosu, Sergei A. Dikanov

Research output: Contribution to journal › Article › peer-review

Abstract

Two-dimensional electron spin-echo envelope modulation (ESEEM) analysis of the uniformly ¹⁵N-labeled archaeal Rieske-type [2Fe-2S] ferredoxin (ARF) from Sulfolobus solfataricus P1 has been conducted in comparison with the previously characterized high-potential protein homologs. Major differences among these proteins were found in the hyperfine sublevel correlation (HYSCORE) lineshapes and intensities of the signals in the (++) quadrant, which are contributed from weakly coupled (non-coordinated) peptide nitrogens near the reduced clusters. They are less pronounced in the HYSCORE spectra of ARF than those of the high-potential protein homologs, and may account for the tuning of Rieske-type clusters in various redox systems.

Original language	English (US)
Pages (from-to)	3467-3472
Number of pages	6
Journal	FEBS Letters
Volume	583
Issue number	21
DOIs	https://doi.org/10.1016/j.febslet.2009.09.050
State	Published - Nov 3 2009
Externally published	Yes

Keywords

Archaea
Electron paramagnetic resonance
Electron spin-echo envelope modulation
Ferredoxin
Hyperfine sublevel correlation
Rieske
[2Fe-2S] cluster

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Online availability

10.1016/j.febslet.2009.09.050

Library availability

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Cite this

@article{6e56fb25fed847709ee5567b8e8d7107,

title = "Two-dimensional pulsed electron spin resonance characterization of 15N-labeled archaeal Rieske-type ferredoxin",

abstract = "Two-dimensional electron spin-echo envelope modulation (ESEEM) analysis of the uniformly 15N-labeled archaeal Rieske-type [2Fe-2S] ferredoxin (ARF) from Sulfolobus solfataricus P1 has been conducted in comparison with the previously characterized high-potential protein homologs. Major differences among these proteins were found in the hyperfine sublevel correlation (HYSCORE) lineshapes and intensities of the signals in the (++) quadrant, which are contributed from weakly coupled (non-coordinated) peptide nitrogens near the reduced clusters. They are less pronounced in the HYSCORE spectra of ARF than those of the high-potential protein homologs, and may account for the tuning of Rieske-type clusters in various redox systems.",

keywords = "Archaea, Electron paramagnetic resonance, Electron spin-echo envelope modulation, Ferredoxin, Hyperfine sublevel correlation, Rieske, [2Fe-2S] cluster",

author = "Toshio Iwasaki and Samoilova, {Rimma I.} and Asako Kounosu and Dikanov, {Sergei A.}",

note = "Funding Information: This work was supported in part by JSPS Grants-in-aid 15770088 , 18608004 and 21659111 (T.I.), by JSPS Grant BSAR-507 (T.I.), by NSF Grant 9910113 (S.A.D.), and by NIH Grant GM62954 (S.A.D.). ",

year = "2009",

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doi = "10.1016/j.febslet.2009.09.050",

language = "English (US)",

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T1 - Two-dimensional pulsed electron spin resonance characterization of 15N-labeled archaeal Rieske-type ferredoxin

AU - Iwasaki, Toshio

AU - Samoilova, Rimma I.

AU - Kounosu, Asako

AU - Dikanov, Sergei A.

N1 - Funding Information: This work was supported in part by JSPS Grants-in-aid 15770088 , 18608004 and 21659111 (T.I.), by JSPS Grant BSAR-507 (T.I.), by NSF Grant 9910113 (S.A.D.), and by NIH Grant GM62954 (S.A.D.).

PY - 2009/11/3

Y1 - 2009/11/3

N2 - Two-dimensional electron spin-echo envelope modulation (ESEEM) analysis of the uniformly 15N-labeled archaeal Rieske-type [2Fe-2S] ferredoxin (ARF) from Sulfolobus solfataricus P1 has been conducted in comparison with the previously characterized high-potential protein homologs. Major differences among these proteins were found in the hyperfine sublevel correlation (HYSCORE) lineshapes and intensities of the signals in the (++) quadrant, which are contributed from weakly coupled (non-coordinated) peptide nitrogens near the reduced clusters. They are less pronounced in the HYSCORE spectra of ARF than those of the high-potential protein homologs, and may account for the tuning of Rieske-type clusters in various redox systems.

AB - Two-dimensional electron spin-echo envelope modulation (ESEEM) analysis of the uniformly 15N-labeled archaeal Rieske-type [2Fe-2S] ferredoxin (ARF) from Sulfolobus solfataricus P1 has been conducted in comparison with the previously characterized high-potential protein homologs. Major differences among these proteins were found in the hyperfine sublevel correlation (HYSCORE) lineshapes and intensities of the signals in the (++) quadrant, which are contributed from weakly coupled (non-coordinated) peptide nitrogens near the reduced clusters. They are less pronounced in the HYSCORE spectra of ARF than those of the high-potential protein homologs, and may account for the tuning of Rieske-type clusters in various redox systems.

KW - Archaea

KW - Electron paramagnetic resonance

KW - Electron spin-echo envelope modulation

KW - Ferredoxin

KW - Hyperfine sublevel correlation

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KW - [2Fe-2S] cluster

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