Two-dimensional gel analysis of polypeptide appearance in forming thylakoid membranes

C. W. Gilbert, D. E. Buetow

Research output: Contribution to journalArticle

Abstract

For the first time, two-dimensional gel electrophoresis is used to analyse the polypeptide composition of thylakoid membranes during chloroplast development. The number of polypeptide spots resolved on the two-dimensional gels is 3-4 times the number of bands previously resolved on one-dimensional gels. The pattern of polypeptide synthesis and insertion into the forming thylakoid membranes of dark-grown Euglena exposed to light conforms with previous biochemical measurements on the assembly of the electron transport chain located in these membranes.

Original languageEnglish (US)
Pages (from-to)649-655
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume107
Issue number2
DOIs
StatePublished - Jul 30 1982

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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