For the first time, two-dimensional gel electrophoresis is used to analyse the polypeptide composition of thylakoid membranes during chloroplast development. The number of polypeptide spots resolved on the two-dimensional gels is 3-4 times the number of bands previously resolved on one-dimensional gels. The pattern of polypeptide synthesis and insertion into the forming thylakoid membranes of dark-grown Euglena exposed to light conforms with previous biochemical measurements on the assembly of the electron transport chain located in these membranes.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jul 30 1982|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology