Tumor necrosis factor α inhibits insulin-like growth factor I-induced hematopoietic cell survival and proliferation

Wen Hong Shen, Jian Hua Zhou, Suzanne R. Broussard, Rodney W. Johnson, Robert Dantzer, Keith W. Kelley

Research output: Contribution to journalArticlepeer-review

Abstract

Proinflammatory cytokines, such as TNFα and IL-1β, are both cytostatic and cytotoxic. In contrast, IGF-I promotes proliferation and survival of hematopoietic progenitor cells. In this report, we establish that both the cytostatic and cytotoxic activity of TNFα on murine myeloid progenitor cells is only evident in the presence of IGF-I. We first confirmed that IGF-I (100 ng/ml) increases DNA synthesis and reduces apoptosis in murine myeloid progenitor cells induced to die by growth factor withdrawal. TNFα inhibits, in a dose-dependent fashion from 0.1 to 10 ng/ml, both activities of IGF-I. TNFα activity was not detected in the absence of IGF-I. Another proinflammatory cytokine, IL-1β, did not inhibit IGF-I-induced activity in murine factor-dependent cell progenitor-1/Mac-1 cells. However, the ability of TNFα to impair IGF-I-induced DNA synthesis in human promyeloid cells extends to IL-1β. Statistically significant inhibition of all these events occurs at very low concentrations of 1 ng/ml or less. These results support the general concept that proinflammatory cytokines impair the actions of hormones on hematopoietic cells, leading to IGF-I receptor resistance.

Original languageEnglish (US)
Pages (from-to)3101-3105
Number of pages5
JournalEndocrinology
Volume145
Issue number7
DOIs
StatePublished - Jul 2004

ASJC Scopus subject areas

  • Endocrinology

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