Truncated HP1 lacking a functional chromodomain induces heterochromatinization upon in vivo targeting

Maartje C. Brink, Yme Van Der Velden, Wim De Leeuw, Julio Mateos-Langerak, Andrew S Belmont, Roel Van Driel, Pernette J. Verschure

Research output: Contribution to journalArticle

Abstract

Packaging of the eukaryotic genome into higher order chromatin structures is tightly related to gene expression. Pericentromeric heterochromatin is typified by accumulations of heterochromatin protein 1 (HP1), methylation of histone H3 at lysine 9 (MeH3K9) and global histone deacetylation. HP1 interacts with chromatin by binding to MeH3K9 through the chromodomain (CD). HP1 dimerizes with itself and binds a variety of proteins through its chromoshadow domain. We have analyzed at the single cell level whether HP1 lacking its functional CD is able to induce heterochromatinization in vivo. We used a lac-operator array-based system in mammalian cells to target EGFP-lac repressor tagged truncated HP1α and HP1β to a lac operator containing gene-amplified chromosome region in living cells. After targeting truncated HP1α or HP1β we observe enhanced tri-MeH3K9 and recruitment of endogenous HP1α and HP1β to the chromosome region. We show that CD-less HP1α can induce chromatin condensation, whereas the effect of truncated HP1β is less pronounced. Our results demonstrate that after lac repressor-mediated targeting, HP1α and HP1β without a functional CD are able to induce heterochromatinization.

Original languageEnglish (US)
Pages (from-to)53-61
Number of pages9
JournalHistochemistry and Cell Biology
Volume125
Issue number1-2
DOIs
StatePublished - Jan 1 2006

Fingerprint

Lac Repressors
Chromatin
heterochromatin-specific nonhistone chromosomal protein HP-1
Histones
Chromosomes
Heterochromatin
Product Packaging
Methylation
Lysine
Genome
Gene Expression
Genes
Proteins

Keywords

  • Epigenetics
  • Gene expression
  • Heterochromatin protein 1
  • Histone methylation
  • Large-scale chromatin structure

ASJC Scopus subject areas

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

Cite this

Brink, M. C., Van Der Velden, Y., De Leeuw, W., Mateos-Langerak, J., Belmont, A. S., Van Driel, R., & Verschure, P. J. (2006). Truncated HP1 lacking a functional chromodomain induces heterochromatinization upon in vivo targeting. Histochemistry and Cell Biology, 125(1-2), 53-61. https://doi.org/10.1007/s00418-005-0088-7

Truncated HP1 lacking a functional chromodomain induces heterochromatinization upon in vivo targeting. / Brink, Maartje C.; Van Der Velden, Yme; De Leeuw, Wim; Mateos-Langerak, Julio; Belmont, Andrew S; Van Driel, Roel; Verschure, Pernette J.

In: Histochemistry and Cell Biology, Vol. 125, No. 1-2, 01.01.2006, p. 53-61.

Research output: Contribution to journalArticle

Brink, MC, Van Der Velden, Y, De Leeuw, W, Mateos-Langerak, J, Belmont, AS, Van Driel, R & Verschure, PJ 2006, 'Truncated HP1 lacking a functional chromodomain induces heterochromatinization upon in vivo targeting', Histochemistry and Cell Biology, vol. 125, no. 1-2, pp. 53-61. https://doi.org/10.1007/s00418-005-0088-7
Brink, Maartje C. ; Van Der Velden, Yme ; De Leeuw, Wim ; Mateos-Langerak, Julio ; Belmont, Andrew S ; Van Driel, Roel ; Verschure, Pernette J. / Truncated HP1 lacking a functional chromodomain induces heterochromatinization upon in vivo targeting. In: Histochemistry and Cell Biology. 2006 ; Vol. 125, No. 1-2. pp. 53-61.
@article{bda7630c747849ef929749cfac8360f4,
title = "Truncated HP1 lacking a functional chromodomain induces heterochromatinization upon in vivo targeting",
abstract = "Packaging of the eukaryotic genome into higher order chromatin structures is tightly related to gene expression. Pericentromeric heterochromatin is typified by accumulations of heterochromatin protein 1 (HP1), methylation of histone H3 at lysine 9 (MeH3K9) and global histone deacetylation. HP1 interacts with chromatin by binding to MeH3K9 through the chromodomain (CD). HP1 dimerizes with itself and binds a variety of proteins through its chromoshadow domain. We have analyzed at the single cell level whether HP1 lacking its functional CD is able to induce heterochromatinization in vivo. We used a lac-operator array-based system in mammalian cells to target EGFP-lac repressor tagged truncated HP1α and HP1β to a lac operator containing gene-amplified chromosome region in living cells. After targeting truncated HP1α or HP1β we observe enhanced tri-MeH3K9 and recruitment of endogenous HP1α and HP1β to the chromosome region. We show that CD-less HP1α can induce chromatin condensation, whereas the effect of truncated HP1β is less pronounced. Our results demonstrate that after lac repressor-mediated targeting, HP1α and HP1β without a functional CD are able to induce heterochromatinization.",
keywords = "Epigenetics, Gene expression, Heterochromatin protein 1, Histone methylation, Large-scale chromatin structure",
author = "Brink, {Maartje C.} and {Van Der Velden}, Yme and {De Leeuw}, Wim and Julio Mateos-Langerak and Belmont, {Andrew S} and {Van Driel}, Roel and Verschure, {Pernette J.}",
year = "2006",
month = "1",
day = "1",
doi = "10.1007/s00418-005-0088-7",
language = "English (US)",
volume = "125",
pages = "53--61",
journal = "Histochemistry and Cell Biology",
issn = "0948-6143",
publisher = "Springer Verlag",
number = "1-2",

}

TY - JOUR

T1 - Truncated HP1 lacking a functional chromodomain induces heterochromatinization upon in vivo targeting

AU - Brink, Maartje C.

AU - Van Der Velden, Yme

AU - De Leeuw, Wim

AU - Mateos-Langerak, Julio

AU - Belmont, Andrew S

AU - Van Driel, Roel

AU - Verschure, Pernette J.

PY - 2006/1/1

Y1 - 2006/1/1

N2 - Packaging of the eukaryotic genome into higher order chromatin structures is tightly related to gene expression. Pericentromeric heterochromatin is typified by accumulations of heterochromatin protein 1 (HP1), methylation of histone H3 at lysine 9 (MeH3K9) and global histone deacetylation. HP1 interacts with chromatin by binding to MeH3K9 through the chromodomain (CD). HP1 dimerizes with itself and binds a variety of proteins through its chromoshadow domain. We have analyzed at the single cell level whether HP1 lacking its functional CD is able to induce heterochromatinization in vivo. We used a lac-operator array-based system in mammalian cells to target EGFP-lac repressor tagged truncated HP1α and HP1β to a lac operator containing gene-amplified chromosome region in living cells. After targeting truncated HP1α or HP1β we observe enhanced tri-MeH3K9 and recruitment of endogenous HP1α and HP1β to the chromosome region. We show that CD-less HP1α can induce chromatin condensation, whereas the effect of truncated HP1β is less pronounced. Our results demonstrate that after lac repressor-mediated targeting, HP1α and HP1β without a functional CD are able to induce heterochromatinization.

AB - Packaging of the eukaryotic genome into higher order chromatin structures is tightly related to gene expression. Pericentromeric heterochromatin is typified by accumulations of heterochromatin protein 1 (HP1), methylation of histone H3 at lysine 9 (MeH3K9) and global histone deacetylation. HP1 interacts with chromatin by binding to MeH3K9 through the chromodomain (CD). HP1 dimerizes with itself and binds a variety of proteins through its chromoshadow domain. We have analyzed at the single cell level whether HP1 lacking its functional CD is able to induce heterochromatinization in vivo. We used a lac-operator array-based system in mammalian cells to target EGFP-lac repressor tagged truncated HP1α and HP1β to a lac operator containing gene-amplified chromosome region in living cells. After targeting truncated HP1α or HP1β we observe enhanced tri-MeH3K9 and recruitment of endogenous HP1α and HP1β to the chromosome region. We show that CD-less HP1α can induce chromatin condensation, whereas the effect of truncated HP1β is less pronounced. Our results demonstrate that after lac repressor-mediated targeting, HP1α and HP1β without a functional CD are able to induce heterochromatinization.

KW - Epigenetics

KW - Gene expression

KW - Heterochromatin protein 1

KW - Histone methylation

KW - Large-scale chromatin structure

UR - http://www.scopus.com/inward/record.url?scp=30044434949&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=30044434949&partnerID=8YFLogxK

U2 - 10.1007/s00418-005-0088-7

DO - 10.1007/s00418-005-0088-7

M3 - Article

C2 - 16283356

AN - SCOPUS:30044434949

VL - 125

SP - 53

EP - 61

JO - Histochemistry and Cell Biology

JF - Histochemistry and Cell Biology

SN - 0948-6143

IS - 1-2

ER -