@inbook{09f4980c3d6d48ee9bef2f35fbb54bd6,
title = "Transposon Insertion Mutagenesis in Hyperthermophilic Crenarchaeon Sulfolobus islandicus",
abstract = "Transposon insertion mutagenesis is a forward genetic approach that has been widely utilized for genetic characterization of bacteria and single-celled eukaryotes, and its applications are being rapidly expanded into a few archaeal model organisms for gene function analysis. Previously, we developed a Tn5-based in vivo transposon insertion mutagenesis system in the hyperthermophilic crenarchaeon S. islandicucs M.16.4 and defined the essential gene set under laboratory growth conditions. In this chapter, we will mainly focus on presenting details regarding the generation of a near-saturating transposon insertion mutant library in this crenarchaeal model. We envision that the traditional transposon-based forward mutagenesis screening paired with next generation sequencing will greatly speed up the exploration of archaeal genomic features.",
keywords = "Archaea, Sulfolobus islandicus, Tn5 transposase, Transposon insertion library, random mutagenesis",
author = "Changyi Zhang and Whitaker, {Rachel J.}",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2445-6_10",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "163--176",
booktitle = "Methods in Molecular Biology",
}