Transporter engineering for cellobiose fermentation under lower pH conditions by engineered Saccharomyces cerevisiae

Eun Joong Oh, Suryang Kwak, Heejin Kim, Yong Su Jin

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this study was to engineer cellodextrin transporter 2 (CDT-2) from Neurospora crassa for improved cellobiose fermentation under lower pH conditions by Saccharomyces cerevisiae. Through directed evolution, a mutant CDT-2 capable of facilitating cellobiose fermentation under lower pH conditions was obtained. Specifically, a library of CDT-2 mutants with GFP fusion was screened by flow cytometry and then serial subcultured to isolate a CDT-2 mutant capable of transporting cellobiose under acidic conditions. The engineered S. cerevisiae expressing the isolated mutant CDT-2 (I96N/T487A) produced ethanol with a specific cellobiose consumption rate of 0.069 g/g cell/h, which was 51% and 55% higher than those of the strains harboring wild-type CDT-1 and CDT-2 in a minimal medium with 2 g/L of acetic acid.

Original languageEnglish (US)
Pages (from-to)1469-1475
Number of pages7
JournalBioresource Technology
Volume245
DOIs
StatePublished - Dec 2017

Keywords

  • Cellobiose
  • Cellodextrin transporter
  • Low pH conditions
  • Saccharomyces cerevisiae
  • Transporter engineering

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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