Transporter engineering for cellobiose fermentation under lower pH conditions by engineered Saccharomyces cerevisiae

Eun Joong Oh; Suryang Kwak; Heejin Kim; Yong Su Jin

doi:10.1016/j.biortech.2017.05.138

Transporter engineering for cellobiose fermentation under lower pH conditions by engineered Saccharomyces cerevisiae

Eun Joong Oh, Suryang Kwak, Heejin Kim, Yong Su Jin

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of this study was to engineer cellodextrin transporter 2 (CDT-2) from Neurospora crassa for improved cellobiose fermentation under lower pH conditions by Saccharomyces cerevisiae. Through directed evolution, a mutant CDT-2 capable of facilitating cellobiose fermentation under lower pH conditions was obtained. Specifically, a library of CDT-2 mutants with GFP fusion was screened by flow cytometry and then serial subcultured to isolate a CDT-2 mutant capable of transporting cellobiose under acidic conditions. The engineered S. cerevisiae expressing the isolated mutant CDT-2 (I96N/T487A) produced ethanol with a specific cellobiose consumption rate of 0.069 g/g cell/h, which was 51% and 55% higher than those of the strains harboring wild-type CDT-1 and CDT-2 in a minimal medium with 2 g/L of acetic acid.

Original language	English (US)
Pages (from-to)	1469-1475
Number of pages	7
Journal	Bioresource Technology
Volume	245
DOIs	https://doi.org/10.1016/j.biortech.2017.05.138
State	Published - Dec 2017

Keywords

Cellobiose
Cellodextrin transporter
Low pH conditions
Saccharomyces cerevisiae
Transporter engineering

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

Online availability

10.1016/j.biortech.2017.05.138

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title = "Transporter engineering for cellobiose fermentation under lower pH conditions by engineered Saccharomyces cerevisiae",

abstract = "The aim of this study was to engineer cellodextrin transporter 2 (CDT-2) from Neurospora crassa for improved cellobiose fermentation under lower pH conditions by Saccharomyces cerevisiae. Through directed evolution, a mutant CDT-2 capable of facilitating cellobiose fermentation under lower pH conditions was obtained. Specifically, a library of CDT-2 mutants with GFP fusion was screened by flow cytometry and then serial subcultured to isolate a CDT-2 mutant capable of transporting cellobiose under acidic conditions. The engineered S. cerevisiae expressing the isolated mutant CDT-2 (I96N/T487A) produced ethanol with a specific cellobiose consumption rate of 0.069 g/g cell/h, which was 51% and 55% higher than those of the strains harboring wild-type CDT-1 and CDT-2 in a minimal medium with 2 g/L of acetic acid.",

keywords = "Cellobiose, Cellodextrin transporter, Low pH conditions, Saccharomyces cerevisiae, Transporter engineering",

author = "Oh, {Eun Joong} and Suryang Kwak and Heejin Kim and Jin, {Yong Su}",

note = "Funding Information: This work was supported by funding from the Energy Biosciences Institute . Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

year = "2017",

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doi = "10.1016/j.biortech.2017.05.138",

language = "English (US)",

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T1 - Transporter engineering for cellobiose fermentation under lower pH conditions by engineered Saccharomyces cerevisiae

AU - Oh, Eun Joong

AU - Kwak, Suryang

AU - Kim, Heejin

AU - Jin, Yong Su

PY - 2017/12

Y1 - 2017/12

N2 - The aim of this study was to engineer cellodextrin transporter 2 (CDT-2) from Neurospora crassa for improved cellobiose fermentation under lower pH conditions by Saccharomyces cerevisiae. Through directed evolution, a mutant CDT-2 capable of facilitating cellobiose fermentation under lower pH conditions was obtained. Specifically, a library of CDT-2 mutants with GFP fusion was screened by flow cytometry and then serial subcultured to isolate a CDT-2 mutant capable of transporting cellobiose under acidic conditions. The engineered S. cerevisiae expressing the isolated mutant CDT-2 (I96N/T487A) produced ethanol with a specific cellobiose consumption rate of 0.069 g/g cell/h, which was 51% and 55% higher than those of the strains harboring wild-type CDT-1 and CDT-2 in a minimal medium with 2 g/L of acetic acid.

AB - The aim of this study was to engineer cellodextrin transporter 2 (CDT-2) from Neurospora crassa for improved cellobiose fermentation under lower pH conditions by Saccharomyces cerevisiae. Through directed evolution, a mutant CDT-2 capable of facilitating cellobiose fermentation under lower pH conditions was obtained. Specifically, a library of CDT-2 mutants with GFP fusion was screened by flow cytometry and then serial subcultured to isolate a CDT-2 mutant capable of transporting cellobiose under acidic conditions. The engineered S. cerevisiae expressing the isolated mutant CDT-2 (I96N/T487A) produced ethanol with a specific cellobiose consumption rate of 0.069 g/g cell/h, which was 51% and 55% higher than those of the strains harboring wild-type CDT-1 and CDT-2 in a minimal medium with 2 g/L of acetic acid.

KW - Cellobiose

KW - Cellodextrin transporter

KW - Low pH conditions

KW - Saccharomyces cerevisiae

KW - Transporter engineering

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VL - 245

SP - 1469

EP - 1475

JO - Agricultural Wastes

JF - Agricultural Wastes

ER -

Transporter engineering for cellobiose fermentation under lower pH conditions by engineered Saccharomyces cerevisiae

Abstract

Keywords

ASJC Scopus subject areas

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