Sealed membrane vesicles were isolated from hypocotyls of two varieties of French bean (Phaseolus vulgaris). The preparations were shown to contain vesicles in which H+ transport and ATPase were sensitive to nitrate but insensitive to vanadate. These sensitivities suggest the vesicles were enriched for tonoplast. Fractions prepared from the α and β races of the fungus Colletotrichum lindemuthianum inhibited H+ transport in the vesicles isolated from cvs. Dark Red Kidney and Great Northern bean tissue. These data are discussed in terms of the biochemical mechanisms operating in plant/pathogen interactions.
- Colletotrichum lindemuthianum
- French bean
- Fungi imperfecti, H transport
- Phaseolus vulgaris, Leguminosae
- membrane ATPase.
ASJC Scopus subject areas
- Molecular Biology
- Plant Science