@article{abc850f4223c49c3bfbc169d8e7868d3,
title = "Transport properties of bean vesicles in the presence of fungal components",
abstract = "Sealed membrane vesicles were isolated from hypocotyls of two varieties of French bean (Phaseolus vulgaris). The preparations were shown to contain vesicles in which H+ transport and ATPase were sensitive to nitrate but insensitive to vanadate. These sensitivities suggest the vesicles were enriched for tonoplast. Fractions prepared from the α and β races of the fungus Colletotrichum lindemuthianum inhibited H+ transport in the vesicles isolated from cvs. Dark Red Kidney and Great Northern bean tissue. These data are discussed in terms of the biochemical mechanisms operating in plant/pathogen interactions.",
keywords = "Colletotrichum lindemuthianum, French bean, Fungi imperfecti, H transport, Phaseolus vulgaris, Leguminosae, membrane ATPase.",
author = "Griffiths, {Helen M.} and Briskin, {Donald P.} and Anderson, {Anne J.}",
note = "Funding Information: Opf~cal measuremtn~s o/ rhe r~srcle pH grodrenr. Proton transport rn membrane vcsicks was measured in the presence of 250 mM sorbitol. 5 mM ATP. 5 mM MgSO,. SO mM KCI. 5 pM qumacnnc. 25 mM bis-trts propane:Ma pH 7.2 and ti mg of membrane protcm. Nitrate (KNO,) when present was at 50 mM and vanadate I Na,VO.) wasa t 50 MM [I R].T hef ungal extracts were used as IOmgl solns and were added to the reactton mixture IO min before commenctng proton transport measurements. Fluorcscemx quenching was reversed by the addttton of 5 PM gramicidm D. The fluorescence measurements were made at room temp. (25{\textquoteright}) with a Pcrkm Elmer LS5 spectrofluonmeter wtth the exatation monochromator se1 at 430nm and the emuston monochromator set at 500 nm Acknowledgemmrs-A J.A. gratefully acknowledges grants from I!SDA-ARS compctitrve grant program and National !kknce Foundation (PCM 0810-5128). The technical asststancc of W R. Thomky IS gratefully acknowledged. WC thank B. Gandy for typing the manuscrtpt. Utah State Agruzultural Expertment Station paper no. 3019",
year = "1986",
month = dec,
day = "23",
doi = "10.1016/S0031-9422(00)81473-9",
language = "English (US)",
volume = "26",
pages = "21--24",
journal = "Phytochemistry",
issn = "0031-9422",
publisher = "Elsevier Limited",
number = "1",
}