Transparent Intracellular Sensing Platform with Si Needles for Simultaneous Live Imaging

Woohyun Park, Eun Mi Kim, Yale Jeon, Junsang Lee, Jonghun Yi, Jinheon Jeong, Bongjoong Kim, Byeong Guk Jeong, Dong Rip Kim, Hyunjoon Kong, Chi Hwan Lee

Research output: Contribution to journalArticlepeer-review


Vertically ordered Si needles are of particular interest for long-term intracellular recording owing to their capacity to infiltrate living cells with negligible damage and minimal toxicity. Such intracellular recordings could greatly benefit from simultaneous live cell imaging without disrupting their culture, contributing to an in-depth understanding of cellular function and activity. However, the use of standard live imaging techniques, such as inverted and confocal microscopy, is currently impeded by the opacity of Si wafers, typically employed for fabricating vertical Si needles. Here, we introduce a transparent intracellular sensing platform that combines vertical Si needles with a percolated network of Au-Ag nanowires on a transparent elastomeric substrate. This sensing platform meets all prerequisites for simultaneous intracellular recording and imaging, including electrochemical impedance, optical transparency, mechanical compliance, and cell viability. Proof-of-concept demonstrations of this sensing platform include monitoring electrical potentials in cardiomyocyte cells and in three-dimensionally engineered cardiovascular tissue, all while conducting live imaging with inverted and confocal microscopes. This sensing platform holds wide-ranging potential applications for intracellular research across various disciplines such as neuroscience, cardiology, muscle physiology, and drug screening.

Original languageEnglish (US)
Pages (from-to)25014-25026
Number of pages13
JournalACS Nano
Issue number24
StatePublished - Dec 26 2023


  • Intracellular recording
  • intraorganoid sensing
  • live cell imaging
  • microneedle
  • nanowires

ASJC Scopus subject areas

  • General Engineering
  • General Materials Science
  • General Physics and Astronomy


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