Transient overexpression of the Miscanthus sinensis glucose-6-phosphate isomerase gene (MsGPI) in Nicotiana benthamiana enhances expression of genes related to antioxidant metabolism

Eun Soo Seong, Ji Hye Yoo, Jae Geun Lee, Hee Young Kim, In Seong Hwang, Kweon Heo, Jung Dae Lim, D. K. Lee, E. J. Sacks, Chang Yeon Yu

Research output: Contribution to journalArticle

Abstract

We investigated the expression of genes related to antioxidant mechanisms through transient overexpression of the glucose-6-phosphate isomerase (GPI) gene of Miscanthus sinensis in Nicotiana benthamiana leaves. Full-length cDNA was isolated from M. sinensis GPI (MsGPI). An analysis of the MsGPI amino acid sequence revealed significant similarities to the GPIs of Festuca ovima (94%), Hordium vulgare (94%), Triticum aestivum (94%), and Zea mays (97%). RT-PCR showed that MsGPI expression was induced significantly by NaCl. Transient M. sinensis GPI sense construct overexpression resulted in increased N. benthamiana ascorbate peroxidase (NbAPX) and phenylalanine ammonia lyase (NbPAL) transcript levels in N. benthamiana leaves. These observations suggest that MsGPI is involved in antioxidant metabolism and that it is a transcriptional regulator of NbAPX and NbPAL.

Original languageEnglish (US)
Pages (from-to)408-414
Number of pages7
JournalPlant OMICS
Volume6
Issue number6
StatePublished - Sep 1 2013

Fingerprint

glucose-6-phosphate isomerase
Miscanthus sinensis
Nicotiana benthamiana
antioxidants
gene expression
metabolism
genes
phenylalanine ammonia-lyase
ascorbate peroxidase
Festuca
leaves
Triticum aestivum
amino acid sequences
transcription factors
Zea mays
reverse transcriptase polymerase chain reaction

Keywords

  • Antioxidant metabolism
  • Glucose-6-phosphate isomerase
  • NbAPX
  • NbPAL
  • Transient overexpression

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Transient overexpression of the Miscanthus sinensis glucose-6-phosphate isomerase gene (MsGPI) in Nicotiana benthamiana enhances expression of genes related to antioxidant metabolism. / Seong, Eun Soo; Yoo, Ji Hye; Lee, Jae Geun; Kim, Hee Young; Hwang, In Seong; Heo, Kweon; Lim, Jung Dae; Lee, D. K.; Sacks, E. J.; Yu, Chang Yeon.

In: Plant OMICS, Vol. 6, No. 6, 01.09.2013, p. 408-414.

Research output: Contribution to journalArticle

Seong, Eun Soo ; Yoo, Ji Hye ; Lee, Jae Geun ; Kim, Hee Young ; Hwang, In Seong ; Heo, Kweon ; Lim, Jung Dae ; Lee, D. K. ; Sacks, E. J. ; Yu, Chang Yeon. / Transient overexpression of the Miscanthus sinensis glucose-6-phosphate isomerase gene (MsGPI) in Nicotiana benthamiana enhances expression of genes related to antioxidant metabolism. In: Plant OMICS. 2013 ; Vol. 6, No. 6. pp. 408-414.
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abstract = "We investigated the expression of genes related to antioxidant mechanisms through transient overexpression of the glucose-6-phosphate isomerase (GPI) gene of Miscanthus sinensis in Nicotiana benthamiana leaves. Full-length cDNA was isolated from M. sinensis GPI (MsGPI). An analysis of the MsGPI amino acid sequence revealed significant similarities to the GPIs of Festuca ovima (94{\%}), Hordium vulgare (94{\%}), Triticum aestivum (94{\%}), and Zea mays (97{\%}). RT-PCR showed that MsGPI expression was induced significantly by NaCl. Transient M. sinensis GPI sense construct overexpression resulted in increased N. benthamiana ascorbate peroxidase (NbAPX) and phenylalanine ammonia lyase (NbPAL) transcript levels in N. benthamiana leaves. These observations suggest that MsGPI is involved in antioxidant metabolism and that it is a transcriptional regulator of NbAPX and NbPAL.",
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AU - Hwang, In Seong

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AB - We investigated the expression of genes related to antioxidant mechanisms through transient overexpression of the glucose-6-phosphate isomerase (GPI) gene of Miscanthus sinensis in Nicotiana benthamiana leaves. Full-length cDNA was isolated from M. sinensis GPI (MsGPI). An analysis of the MsGPI amino acid sequence revealed significant similarities to the GPIs of Festuca ovima (94%), Hordium vulgare (94%), Triticum aestivum (94%), and Zea mays (97%). RT-PCR showed that MsGPI expression was induced significantly by NaCl. Transient M. sinensis GPI sense construct overexpression resulted in increased N. benthamiana ascorbate peroxidase (NbAPX) and phenylalanine ammonia lyase (NbPAL) transcript levels in N. benthamiana leaves. These observations suggest that MsGPI is involved in antioxidant metabolism and that it is a transcriptional regulator of NbAPX and NbPAL.

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