Transient overexpression of the Miscanthus sinensis glucose-6-phosphate isomerase gene (MsGPI) in Nicotiana benthamiana enhances expression of genes related to antioxidant metabolism

Eun Soo Seong; Ji Hye Yoo; Jae Geun Lee; Hee Young Kim; In Seong Hwang; Kweon Heo; Jung Dae Lim; D. K. Lee; E. J. Sacks; Chang Yeon Yu

Transient overexpression of the Miscanthus sinensis glucose-6-phosphate isomerase gene (MsGPI) in Nicotiana benthamiana enhances expression of genes related to antioxidant metabolism

Eun Soo Seong, Ji Hye Yoo, Jae Geun Lee, Hee Young Kim, In Seong Hwang, Kweon Heo, Jung Dae Lim, D. K. Lee, E. J. Sacks, Chang Yeon Yu

Research output: Contribution to journal › Article › peer-review

Abstract

We investigated the expression of genes related to antioxidant mechanisms through transient overexpression of the glucose-6-phosphate isomerase (GPI) gene of Miscanthus sinensis in Nicotiana benthamiana leaves. Full-length cDNA was isolated from M. sinensis GPI (MsGPI). An analysis of the MsGPI amino acid sequence revealed significant similarities to the GPIs of Festuca ovima (94%), Hordium vulgare (94%), Triticum aestivum (94%), and Zea mays (97%). RT-PCR showed that MsGPI expression was induced significantly by NaCl. Transient M. sinensis GPI sense construct overexpression resulted in increased N. benthamiana ascorbate peroxidase (NbAPX) and phenylalanine ammonia lyase (NbPAL) transcript levels in N. benthamiana leaves. These observations suggest that MsGPI is involved in antioxidant metabolism and that it is a transcriptional regulator of NbAPX and NbPAL.

Original language	English (US)
Pages (from-to)	408-414
Number of pages	7
Journal	Plant OMICS
Volume	6
Issue number	6
State	Published - Sep 2013

Keywords

Antioxidant metabolism
Glucose-6-phosphate isomerase
NbAPX
NbPAL
Transient overexpression

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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title = "Transient overexpression of the Miscanthus sinensis glucose-6-phosphate isomerase gene (MsGPI) in Nicotiana benthamiana enhances expression of genes related to antioxidant metabolism",

abstract = "We investigated the expression of genes related to antioxidant mechanisms through transient overexpression of the glucose-6-phosphate isomerase (GPI) gene of Miscanthus sinensis in Nicotiana benthamiana leaves. Full-length cDNA was isolated from M. sinensis GPI (MsGPI). An analysis of the MsGPI amino acid sequence revealed significant similarities to the GPIs of Festuca ovima (94%), Hordium vulgare (94%), Triticum aestivum (94%), and Zea mays (97%). RT-PCR showed that MsGPI expression was induced significantly by NaCl. Transient M. sinensis GPI sense construct overexpression resulted in increased N. benthamiana ascorbate peroxidase (NbAPX) and phenylalanine ammonia lyase (NbPAL) transcript levels in N. benthamiana leaves. These observations suggest that MsGPI is involved in antioxidant metabolism and that it is a transcriptional regulator of NbAPX and NbPAL.",

keywords = "Antioxidant metabolism, Glucose-6-phosphate isomerase, NbAPX, NbPAL, Transient overexpression",

author = "Seong, {Eun Soo} and Yoo, {Ji Hye} and Lee, {Jae Geun} and Kim, {Hee Young} and Hwang, {In Seong} and Kweon Heo and Lim, {Jung Dae} and Lee, {D. K.} and Sacks, {E. J.} and Yu, {Chang Yeon}",

year = "2013",

month = sep,

language = "English (US)",

volume = "6",

pages = "408--414",

journal = "Plant OMICS",

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number = "6",

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T1 - Transient overexpression of the Miscanthus sinensis glucose-6-phosphate isomerase gene (MsGPI) in Nicotiana benthamiana enhances expression of genes related to antioxidant metabolism

AU - Seong, Eun Soo

AU - Yoo, Ji Hye

AU - Lee, Jae Geun

AU - Kim, Hee Young

AU - Hwang, In Seong

AU - Heo, Kweon

AU - Lim, Jung Dae

AU - Lee, D. K.

AU - Sacks, E. J.

AU - Yu, Chang Yeon

PY - 2013/9

Y1 - 2013/9

N2 - We investigated the expression of genes related to antioxidant mechanisms through transient overexpression of the glucose-6-phosphate isomerase (GPI) gene of Miscanthus sinensis in Nicotiana benthamiana leaves. Full-length cDNA was isolated from M. sinensis GPI (MsGPI). An analysis of the MsGPI amino acid sequence revealed significant similarities to the GPIs of Festuca ovima (94%), Hordium vulgare (94%), Triticum aestivum (94%), and Zea mays (97%). RT-PCR showed that MsGPI expression was induced significantly by NaCl. Transient M. sinensis GPI sense construct overexpression resulted in increased N. benthamiana ascorbate peroxidase (NbAPX) and phenylalanine ammonia lyase (NbPAL) transcript levels in N. benthamiana leaves. These observations suggest that MsGPI is involved in antioxidant metabolism and that it is a transcriptional regulator of NbAPX and NbPAL.

AB - We investigated the expression of genes related to antioxidant mechanisms through transient overexpression of the glucose-6-phosphate isomerase (GPI) gene of Miscanthus sinensis in Nicotiana benthamiana leaves. Full-length cDNA was isolated from M. sinensis GPI (MsGPI). An analysis of the MsGPI amino acid sequence revealed significant similarities to the GPIs of Festuca ovima (94%), Hordium vulgare (94%), Triticum aestivum (94%), and Zea mays (97%). RT-PCR showed that MsGPI expression was induced significantly by NaCl. Transient M. sinensis GPI sense construct overexpression resulted in increased N. benthamiana ascorbate peroxidase (NbAPX) and phenylalanine ammonia lyase (NbPAL) transcript levels in N. benthamiana leaves. These observations suggest that MsGPI is involved in antioxidant metabolism and that it is a transcriptional regulator of NbAPX and NbPAL.

KW - Antioxidant metabolism

KW - Glucose-6-phosphate isomerase

KW - NbAPX

KW - NbPAL

KW - Transient overexpression

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M3 - Article

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VL - 6

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JO - Plant OMICS

JF - Plant OMICS

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ER -

Transient overexpression of the Miscanthus sinensis glucose-6-phosphate isomerase gene (MsGPI) in Nicotiana benthamiana enhances expression of genes related to antioxidant metabolism

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