Transient heat release during induced mitochondrial proton uncoupling

Manjunath C. Rajagopal, Jeffrey W. Brown, Dhruv Gelda, Krishna V. Valavala, Huan Wang, Daniel Adolfo Llano, Rhanor Gillette, Sanjiv Sinha

Research output: Contribution to journalArticle

Abstract

Non-shivering thermogenesis through mitochondrial proton uncoupling is one of the dominant thermoregulatory mechanisms crucial for normal cellular functions. The metabolic pathway for intracellular temperature rise has widely been considered as steady-state substrate oxidation. Here, we show that a transient proton motive force (pmf) dissipation is more dominant than steady-state substrate oxidation in stimulated thermogenesis. Using transient intracellular thermometry during stimulated proton uncoupling in neurons of Aplysia californica, we observe temperature spikes of ~7.5 K that decay over two time scales: a rapid decay of ~4.8 K over ~1 s followed by a slower decay over ~17 s. The rapid decay correlates well in time with transient electrical heating from proton transport across the mitochondrial inner membrane. Beyond ~33 s, we do not observe any heating from intracellular sources, including substrate oxidation and pmf dissipation. Our measurements demonstrate the utility of transient thermometry in better understanding the thermochemistry of mitochondrial metabolism.

Original languageEnglish (US)
Article number279
JournalCommunications biology
Volume2
Issue number1
DOIs
StatePublished - 2019

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Thermometry
protons
Protons
Proton-Motive Force
Thermogenesis
Hot Temperature
deterioration
heat
Heating
oxidation
heat production
Aplysia
Temperature
Oxidation
Mitochondrial Membranes
Metabolic Networks and Pathways
Substrates
Thermochemistry
biochemical pathways
Neurons

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Medicine (miscellaneous)

Cite this

Transient heat release during induced mitochondrial proton uncoupling. / Rajagopal, Manjunath C.; Brown, Jeffrey W.; Gelda, Dhruv; Valavala, Krishna V.; Wang, Huan; Llano, Daniel Adolfo; Gillette, Rhanor; Sinha, Sanjiv.

In: Communications biology, Vol. 2, No. 1, 279, 2019.

Research output: Contribution to journalArticle

Rajagopal, Manjunath C. ; Brown, Jeffrey W. ; Gelda, Dhruv ; Valavala, Krishna V. ; Wang, Huan ; Llano, Daniel Adolfo ; Gillette, Rhanor ; Sinha, Sanjiv. / Transient heat release during induced mitochondrial proton uncoupling. In: Communications biology. 2019 ; Vol. 2, No. 1.
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