TY - JOUR
T1 - Transcriptomics and iTRAQ-Proteomics Analyses of Bovine Mammary Tissue with Streptococcus agalactiae-Induced Mastitis
AU - Zhang, Huimin
AU - Jiang, Hongrui
AU - Fan, Yongliang
AU - Chen, Zhi
AU - Li, Mingxun
AU - Mao, Yongjiang
AU - Karrow, Niel A.
AU - Loor, Juan J.
AU - Moore, Stephen
AU - Yang, Zhangping
N1 - Funding Information:
*E-mail: yzp@yzu.edu.cn. Phone: +86-514-8797-9307. Fax: +86-514-8735−0440. ORCID Huimin Zhang: 0000-0002-8547-8243 Zhi Chen: 0000-0003-3924-1891 Funding This research was supported by the National Natural Science Foundation of China (31472067, 31702142, 31872324), Natural Science Foundation of Jiangsu Province of China (BK20160455), Jiangsu Planned Projects for Postdoctoral Research Funds (1501118B), The Natural Science Foundation of the Jiangsu Higher Education Institutions of China (18KJA230003) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). Notes The authors declare no competing financial interest.
PY - 2018/10/24
Y1 - 2018/10/24
N2 - Mastitis is a highly prevalent disease in dairy cows that causes large economic losses. Streptococcus agalactiae is a common contagious pathogen and a major cause of bovine mastitis. The immune response to intramammary infection with S. agalactiae in dairy cows is a very complex biological process. To understand the host immune response to S. agalactiae-induced mastitis, mammary gland of lactating Chinese Holstein cows was challenged with S. agalactiae via nipple tube perfusion. Visual inspection, analysis of milk somatic cell counts, histopathology, and transmission electron microscopy of mammary tissue were performed to confirm S. agalactiae-induced mastitis. Microarray and isobaric tags for relative and absolute quantitation (iTRAQ) were used to compare the transcriptomes and proteomes of healthy and mastitic mammary tissue. Compared with healthy tissue, a total of 129 differentially expressed genes (DEGs, fold change >2, p < 0.05) and 144 differentially expressed proteins (DEPs, fold change >1.2, p < 0.05) were identified in mammary tissue from S. agalactiae-challenged cows. Among the concordant 18 DEGs/DEPs, immunoglobulin M precursor, cathelicidin-7 precursor, integrin alpha-5, and complement C4-A-like isoform X1 were associated with mastitis. Intramammary infection with S. agalactiae triggered a complex host innate immune response that involved complement and coagulation cascades, ECM-receptor interaction, focal adhesion, and phagosome and bacterial invasion of epithelial cells pathways. These results provide candidate genes or proteins for further studies in the context of prevention and targeted treatment of bovine mastitis.
AB - Mastitis is a highly prevalent disease in dairy cows that causes large economic losses. Streptococcus agalactiae is a common contagious pathogen and a major cause of bovine mastitis. The immune response to intramammary infection with S. agalactiae in dairy cows is a very complex biological process. To understand the host immune response to S. agalactiae-induced mastitis, mammary gland of lactating Chinese Holstein cows was challenged with S. agalactiae via nipple tube perfusion. Visual inspection, analysis of milk somatic cell counts, histopathology, and transmission electron microscopy of mammary tissue were performed to confirm S. agalactiae-induced mastitis. Microarray and isobaric tags for relative and absolute quantitation (iTRAQ) were used to compare the transcriptomes and proteomes of healthy and mastitic mammary tissue. Compared with healthy tissue, a total of 129 differentially expressed genes (DEGs, fold change >2, p < 0.05) and 144 differentially expressed proteins (DEPs, fold change >1.2, p < 0.05) were identified in mammary tissue from S. agalactiae-challenged cows. Among the concordant 18 DEGs/DEPs, immunoglobulin M precursor, cathelicidin-7 precursor, integrin alpha-5, and complement C4-A-like isoform X1 were associated with mastitis. Intramammary infection with S. agalactiae triggered a complex host innate immune response that involved complement and coagulation cascades, ECM-receptor interaction, focal adhesion, and phagosome and bacterial invasion of epithelial cells pathways. These results provide candidate genes or proteins for further studies in the context of prevention and targeted treatment of bovine mastitis.
KW - Streptococcus agalactiae
KW - bovine mastitis
KW - iTRAQ proteome
KW - innate immune response
KW - transcriptome
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U2 - 10.1021/acs.jafc.8b02386
DO - 10.1021/acs.jafc.8b02386
M3 - Article
C2 - 30096236
AN - SCOPUS:85052295259
SN - 0021-8561
VL - 66
SP - 11188
EP - 11196
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 42
ER -