Transcriptomic profiles of human foreskin fibroblast cells in response to orf virus

Daxiang Chen, Mingjian Long, Bin Xiao, Yufeng Xiong, Huiqin Chen, Yu Chen, Zhenzhan Kuang, Ming Li, Yingsong Wu, Daniel L. Rock, Daoyuan Gong, Yong Wang, Haijian He, Fang Liu, Shuhong Luo, Wenbo Hao

Research output: Contribution to journalArticlepeer-review

Abstract

Orf virus has been utilized as a safe and efficient viral vector against not only diverse infectious diseases, but also against tumors. However, the nature of the genes triggered by the vector in human cells is poorly characterized. Using RNA sequencing technology, we compared specific changes in the transcriptomic profiles in human foreskin fibroblast cells following infection by the orf virus. The results indicated that orf virus upregulates or downregulates expression of a variety of genes, including genes involved in antiviral immune response, apoptosis, cell cycle and a series of signaling pathways, such as the IFN and p53-signaling pathways. The orf virus stimulates or inhibits immune gene expression such as chemokines, chemokine receptors, cytokines, cytokine receptors, and molecules involved in antigen uptake and processing after infection. Expression of pro-apoptotic genes increased at 8 hours post-infection. The p53 signaling pathway was activated to induce apoptosis at the same time. However, the cell cycle program was promoted after infection, which may be due to the immunomodulatory genes of the orf virus. This presents the first description of transcription profile changes in human foreskin fibroblast cells after orf virus infection and provides an in-depth analysis of the interaction between the host and orf virus. These data offer new insights into the understanding of the mechanisms of infection by orf virus and identify potential targets for future studies.

Original languageEnglish (US)
Pages (from-to)58668-58685
Number of pages18
JournalOncotarget
Volume8
Issue number35
DOIs
StatePublished - 2017

Keywords

  • Antiviral immune response
  • Apoptosis
  • Cell cycle
  • Orf virus
  • Transcriptomic profiles

ASJC Scopus subject areas

  • Oncology

Fingerprint Dive into the research topics of 'Transcriptomic profiles of human foreskin fibroblast cells in response to orf virus'. Together they form a unique fingerprint.

Cite this