Transcriptome profiling revealed stress-induced and disease resistance genes up-regulated in PRSV resistant transgenic papaya

Jingping Fang, Aiting Lin, Weijing Qiu, Hanyang Cai, Muhammad Umar, Rukai Chen, Ray Ming

Research output: Contribution to journalArticle

Abstract

Papaya is a productive and nutritious tropical fruit. Papaya Ringspot Virus (PRSV) is the most devastating pathogen threatening papaya production worldwide. Development of transgenic resistant varieties is the most effective strategy to control this disease. However, little is known about the genome-wide functional changes induced by particle bombardment transformation. We conducted transcriptome sequencing of PRSV resistant transgenic papaya SunUp and its PRSV susceptible progenitor Sunset to compare the transcriptional changes in young healthy leaves prior to infection with PRSV. In total, 20,700 transcripts were identified, and 842 differentially expressed genes (DEGs) randomly distributed among papaya chromosomes. Gene ontology (GO) category analysis revealed that microtubule-related categories were highly enriched among these DEGs. Numerous DEGs related to various transcription factors, transporters and hormone biosynthesis showed clear differences between the two cultivars, and most were up-regulated in transgenic papaya. Many known and novel stress-induced and disease-resistance genes were most highly expressed in SunUp, including MYB, WRKY, ERF, NAC, nitrate and zinc transporters, and genes involved in the abscisic acid, salicylic acid, and ethylene signaling pathways. We also identified 67,686 alternative splicing (AS) events in Sunset and 68,455 AS events in SunUp, mapping to 10,994 and 10,995 papaya annotated genes, respectively. GO enrichment for the genes displaying AS events exclusively in Sunset was significantly different from those in SunUp. Transcriptomes in Sunset and transgenic SunUp are very similar with noteworthy differences, which increased PRSV-resistance in transgenic papaya. No detrimental pathways and allergenic or toxic proteins were induced on a genome-wide scale in transgenic SunUp. Our results provide a foundation for unraveling the mechanism of PRSV resistance in transgenic papaya.

Original languageEnglish (US)
Article number855
JournalFrontiers in Plant Science
Volume7
Issue numberJUNE2016
DOIs
StatePublished - Jun 16 2016

Fingerprint

Papaya ringspot virus
induced resistance
papayas
transcriptomics
disease resistance
genetically modified organisms
genes
alternative splicing
transporters
genome
tropical and subtropical fruits
salicylic acid
transcriptome
microtubules
abscisic acid
ethylene
disease control
transcription factors
zinc
nitrates

Keywords

  • Alternative splicing
  • Carica papaya L
  • Differentially expressed gene
  • Papaya ringspot virus (PRSV)
  • Transgenic papaya

ASJC Scopus subject areas

  • Plant Science

Cite this

Transcriptome profiling revealed stress-induced and disease resistance genes up-regulated in PRSV resistant transgenic papaya. / Fang, Jingping; Lin, Aiting; Qiu, Weijing; Cai, Hanyang; Umar, Muhammad; Chen, Rukai; Ming, Ray.

In: Frontiers in Plant Science, Vol. 7, No. JUNE2016, 855, 16.06.2016.

Research output: Contribution to journalArticle

Fang, Jingping ; Lin, Aiting ; Qiu, Weijing ; Cai, Hanyang ; Umar, Muhammad ; Chen, Rukai ; Ming, Ray. / Transcriptome profiling revealed stress-induced and disease resistance genes up-regulated in PRSV resistant transgenic papaya. In: Frontiers in Plant Science. 2016 ; Vol. 7, No. JUNE2016.
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