TY - JOUR
T1 - Transcriptome identification of putative genes involved in protein catabolism and innate immune response in human body louse (Pediculicidae: Pediculus humanus)
AU - Pedra, Joao H.F.
AU - Brandt, Amanda
AU - Li, Hong Mei
AU - Westerman, Rick
AU - Romero-Severson, Jeanne
AU - Pollack, Richard J.
AU - Murdock, Larry L.
AU - Pittendrigh, Barry R.
AU - Collins, F. H.
N1 - Funding Information:
Dr Robin Todd of Insect Control and Research Inc., Baltimore, MD generously provided frozen engorged adult human body lice. The authors are grateful to Dr Philip San Miguel and Mr Paul Parker for sequencing all the clones and Mr Swapnal Shah for helping with the database analysis. This manuscript is a publication of the Indiana Center for Insect Genomics (ICIG), a consortium of investigators at the University of Notre Dame and Purdue University. ICIG is supported by a grant from the State of Indiana 21st Century Fund as well as by matching resources from the University of Notre Dame and Purdue University. ICIG Director and grant PI is F.H. Collins of the University of Notre Dame; grant Co-PI is J. Romero-Severson of Purdue University. This project is part of the MPRINT program and was supported by the Department of Entomology funds to BRP. The Purdue Research Foundation supported JHFP and a Ross Graduate Fellowship supported H-ML. This is publication No. 17114, Purdue University Agricultural Experimental Station, West Lafayette, IN, USA.
PY - 2003/11
Y1 - 2003/11
N2 - Genomics information relating to human body lice is surprisingly scarce, and this has constrained studies of their physiology, immunology and vector biology. To identify novel body louse genes, we used engorged adult lice to generate a cDNA library. Initially, 1152 clones were screened for inserts, edited for removal of vector sequences and base pairs of poor quality, and viewed for splicing variations, gene families and polymorphism. Computational methods identified 506 inferred open reading frames including the first predicted louse defensin. The inferred defensin aligns well with other insect defensins and has highly conserved cysteine residues, as are known for other defensin sequences. Two cysteine and five serine proteinases were categorized according to their inferred catalytic sites. We also discovered seven putative ubiquitin-pathway genes and four iron metabolizing deduced enzymes. Finally, glutathione-S-transferases and cytochrome P450 genes were among the detoxification enzymes found. Results from this first systematic effort to discover human body louse genes should promote further studies in Phthiraptera and lice.
AB - Genomics information relating to human body lice is surprisingly scarce, and this has constrained studies of their physiology, immunology and vector biology. To identify novel body louse genes, we used engorged adult lice to generate a cDNA library. Initially, 1152 clones were screened for inserts, edited for removal of vector sequences and base pairs of poor quality, and viewed for splicing variations, gene families and polymorphism. Computational methods identified 506 inferred open reading frames including the first predicted louse defensin. The inferred defensin aligns well with other insect defensins and has highly conserved cysteine residues, as are known for other defensin sequences. Two cysteine and five serine proteinases were categorized according to their inferred catalytic sites. We also discovered seven putative ubiquitin-pathway genes and four iron metabolizing deduced enzymes. Finally, glutathione-S-transferases and cytochrome P450 genes were among the detoxification enzymes found. Results from this first systematic effort to discover human body louse genes should promote further studies in Phthiraptera and lice.
KW - Expressed sequence tag
KW - Functional genomics
KW - Human lice
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U2 - 10.1016/S0965-1748(03)00133-4
DO - 10.1016/S0965-1748(03)00133-4
M3 - Article
C2 - 14563364
AN - SCOPUS:0242307126
SN - 0965-1748
VL - 33
SP - 1135
EP - 1143
JO - Insect Biochemistry and Molecular Biology
JF - Insect Biochemistry and Molecular Biology
IS - 11
ER -