Transcriptome analysis of the cowpea weevil bruchid: Identification of putative proteinases and α-amylases associated with food breakdown

J. H.F. Pedra, A. Brandt, R. Westerman, N. Lobo, H. M. Li, J. Romero-Severson, L. L. Murdock, Barry Robert Pittendrigh

Research output: Contribution to journalArticlepeer-review

Abstract

We describe here the first systematic work to discover insect genes involved in food breakdown using a cDNA library enriched for gut-expressed transcripts from Callosobruchus maculatus. A total of 1056 clones were screened for cDNA insert-containing plasmids, and 503 nonredundant open reading frames were discovered. Twenty-three inferred genes potentially involved in digestive processes in cowpea weevil were identified, including proteinases and amylases. The predicted catalytic sites were identified in the inferred cysteine and aspartic acid proteinases, and in α-amylases. Transcriptome analysis of the cowpea bruchid will potentially permit gene discovery in other beetles, an insect order of major economic and ecological importance that is poorly represented in genomic databases.

Original languageEnglish (US)
Pages (from-to)405-412
Number of pages8
JournalInsect Molecular Biology
Volume12
Issue number4
DOIs
StatePublished - Aug 1 2003

Keywords

  • Callosobruchus maculatus
  • Digestive enzymes
  • Expressed sequence tag
  • Insect gut

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science

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