Transcription Factor RFX2 Is a Key Regulator of Mouse Spermiogenesis

Yujian Wu; Xiangjing Hu; Zhen Li; Min Wang; Sisi Li; Xiuxia Wang; Xiwen Lin; Shangying Liao; Zhuqiang Zhang; Xue Feng; Si Wang; Xiuhong Cui; Yanling Wang; Fei Gao; Rex A. Hess; Chunsheng Han

doi:10.1038/srep20435

Transcription Factor RFX2 Is a Key Regulator of Mouse Spermiogenesis

Yujian Wu, Xiangjing Hu, Zhen Li, Min Wang, Sisi Li, Xiuxia Wang, Xiwen Lin, Shangying Liao, Zhuqiang Zhang, Xue Feng, Si Wang, Xiuhong Cui, Yanling Wang, Fei Gao, Rex A. Hess, Chunsheng Han

Comparative Biosciences

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Abstract

The regulatory factor X (RFX) family of transcription factors is crucial for ciliogenesis throughout evolution. In mice, Rfx1-4 are highly expressed in the testis where flagellated sperm are produced, but the functions of these factors in spermatogenesis remain unknown. Here, we report the production and characterization of the Rfx2 knockout mice. The male knockout mice were sterile due to the arrest of spermatogenesis at an early round spermatid step. The Rfx2-null round spermatids detached from the seminiferous tubules, forming large multinucleated giant cells that underwent apoptosis. In the mutants, formation of the flagellum was inhibited at its earliest stage. RNA-seq analysis identified a large number of cilia-related genes and testis-specific genes that were regulated by RFX2. Many of these genes were direct targets of RFX2, as revealed by chromatin immunoprecipitation-PCR assays. These findings indicate that RFX2 is a key regulator of the post-meiotic development of mouse spermatogenic cells.

Original language	English (US)
Article number	20435
Journal	Scientific reports
Volume	6
DOIs	https://doi.org/10.1038/srep20435
State	Published - Feb 8 2016

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Transcription Factor RFX2 Is a Key Regulator of Mouse Spermiogenesis. / Wu, Yujian; Hu, Xiangjing; Li, Zhen; Wang, Min; Li, Sisi; Wang, Xiuxia; Lin, Xiwen; Liao, Shangying; Zhang, Zhuqiang; Feng, Xue; Wang, Si; Cui, Xiuhong; Wang, Yanling; Gao, Fei; Hess, Rex A.; Han, Chunsheng.

In: Scientific reports, Vol. 6, 20435, 08.02.2016.

Research output: Contribution to journal › Article › peer-review

@article{201695f6ea20454b8864975b04f1850e,

title = "Transcription Factor RFX2 Is a Key Regulator of Mouse Spermiogenesis",

abstract = "The regulatory factor X (RFX) family of transcription factors is crucial for ciliogenesis throughout evolution. In mice, Rfx1-4 are highly expressed in the testis where flagellated sperm are produced, but the functions of these factors in spermatogenesis remain unknown. Here, we report the production and characterization of the Rfx2 knockout mice. The male knockout mice were sterile due to the arrest of spermatogenesis at an early round spermatid step. The Rfx2-null round spermatids detached from the seminiferous tubules, forming large multinucleated giant cells that underwent apoptosis. In the mutants, formation of the flagellum was inhibited at its earliest stage. RNA-seq analysis identified a large number of cilia-related genes and testis-specific genes that were regulated by RFX2. Many of these genes were direct targets of RFX2, as revealed by chromatin immunoprecipitation-PCR assays. These findings indicate that RFX2 is a key regulator of the post-meiotic development of mouse spermatogenic cells.",

author = "Yujian Wu and Xiangjing Hu and Zhen Li and Min Wang and Sisi Li and Xiuxia Wang and Xiwen Lin and Shangying Liao and Zhuqiang Zhang and Xue Feng and Si Wang and Xiuhong Cui and Yanling Wang and Fei Gao and Hess, {Rex A.} and Chunsheng Han",

note = "Funding Information: This work was supported by grants from the Ministry of Science and Technology of China (2013CB945001 and 2012CB966702), National Natural Science Foundation of China (31271379, 31471349). We thank Prof. Yi-Xun Liu for the AFAF antibody and Ms. Hua Qin and Shi-Wen Li for help with image analysis.",

year = "2016",

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day = "8",

doi = "10.1038/srep20435",

language = "English (US)",

volume = "6",

journal = "Scientific Reports",

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publisher = "Nature Publishing Group",

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T1 - Transcription Factor RFX2 Is a Key Regulator of Mouse Spermiogenesis

AU - Wu, Yujian

AU - Hu, Xiangjing

AU - Li, Zhen

AU - Wang, Min

AU - Li, Sisi

AU - Wang, Xiuxia

AU - Lin, Xiwen

AU - Liao, Shangying

AU - Zhang, Zhuqiang

AU - Feng, Xue

AU - Wang, Si

AU - Cui, Xiuhong

AU - Wang, Yanling

AU - Gao, Fei

AU - Hess, Rex A.

AU - Han, Chunsheng

N1 - Funding Information: This work was supported by grants from the Ministry of Science and Technology of China (2013CB945001 and 2012CB966702), National Natural Science Foundation of China (31271379, 31471349). We thank Prof. Yi-Xun Liu for the AFAF antibody and Ms. Hua Qin and Shi-Wen Li for help with image analysis.

PY - 2016/2/8

Y1 - 2016/2/8

N2 - The regulatory factor X (RFX) family of transcription factors is crucial for ciliogenesis throughout evolution. In mice, Rfx1-4 are highly expressed in the testis where flagellated sperm are produced, but the functions of these factors in spermatogenesis remain unknown. Here, we report the production and characterization of the Rfx2 knockout mice. The male knockout mice were sterile due to the arrest of spermatogenesis at an early round spermatid step. The Rfx2-null round spermatids detached from the seminiferous tubules, forming large multinucleated giant cells that underwent apoptosis. In the mutants, formation of the flagellum was inhibited at its earliest stage. RNA-seq analysis identified a large number of cilia-related genes and testis-specific genes that were regulated by RFX2. Many of these genes were direct targets of RFX2, as revealed by chromatin immunoprecipitation-PCR assays. These findings indicate that RFX2 is a key regulator of the post-meiotic development of mouse spermatogenic cells.

AB - The regulatory factor X (RFX) family of transcription factors is crucial for ciliogenesis throughout evolution. In mice, Rfx1-4 are highly expressed in the testis where flagellated sperm are produced, but the functions of these factors in spermatogenesis remain unknown. Here, we report the production and characterization of the Rfx2 knockout mice. The male knockout mice were sterile due to the arrest of spermatogenesis at an early round spermatid step. The Rfx2-null round spermatids detached from the seminiferous tubules, forming large multinucleated giant cells that underwent apoptosis. In the mutants, formation of the flagellum was inhibited at its earliest stage. RNA-seq analysis identified a large number of cilia-related genes and testis-specific genes that were regulated by RFX2. Many of these genes were direct targets of RFX2, as revealed by chromatin immunoprecipitation-PCR assays. These findings indicate that RFX2 is a key regulator of the post-meiotic development of mouse spermatogenic cells.

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Transcription Factor RFX2 Is a Key Regulator of Mouse Spermiogenesis

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