Tracking the binding of multi-functional fluorescent tags for Alzheimer's disease using quantitative multiphoton microscopy

Rishyashring R. Iyer, Carlos A. Renteria, Lingxiao Yang, Janet E. Sorrells, Jaena Park, Liang Sun, Zhengxin Yu, Yiran Huang, Marina Marjanovic, Liviu M. Mirica, Stephen A. Boppart

Research output: Contribution to journalArticlepeer-review

Abstract

A recent theranostic approach to address Alzheimer's disease (AD) utilizes multifunctional targets that both tag and negate the toxicity of AD biomarkers. These compounds, which emit fluorescence with both an activation and a spectral shift in the presence of Aβ, were previously characterized with traditional fluorescence imaging for binary characterization. However, these multifunctional compounds have broad and dynamic emission spectra that are dependent on factors such as the local environment, presence of Aβ deposits, etc. Since quantitative multiphoton microscopy is sensitive to the binding dynamics of molecules, we characterized the performance of two such compounds, LS-4 and ZY-12-OMe, using Simultaneous Label-free Autofluorescence Multi-harmonic (SLAM) microscopy and Fast Optical Coherence, Autofluorescence Lifetime imaging and Second harmonic generation (FOCALS) microscopy. This study shows that the combination of quantitative multiphoton imaging with multifunctional tags for AD offers new insights into the interaction of these tags with AD biomarkers and the theranostic mechanisms.

Original languageEnglish (US)
Article numbere202200105
JournalJournal of Biophotonics
Volume15
Issue number9
DOIs
StatePublished - Sep 2022

Keywords

  • Alzheimer's disease
  • multimodal imaging
  • multiphoton microscopy
  • optical imaging
  • theranostics

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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