@article{65f9c72cadd442e2bb293d1a9c99e89c,
title = "Tracking the binding of multi-functional fluorescent tags for Alzheimer's disease using quantitative multiphoton microscopy",
abstract = "A recent theranostic approach to address Alzheimer's disease (AD) utilizes multifunctional targets that both tag and negate the toxicity of AD biomarkers. These compounds, which emit fluorescence with both an activation and a spectral shift in the presence of Aβ, were previously characterized with traditional fluorescence imaging for binary characterization. However, these multifunctional compounds have broad and dynamic emission spectra that are dependent on factors such as the local environment, presence of Aβ deposits, etc. Since quantitative multiphoton microscopy is sensitive to the binding dynamics of molecules, we characterized the performance of two such compounds, LS-4 and ZY-12-OMe, using Simultaneous Label-free Autofluorescence Multi-harmonic (SLAM) microscopy and Fast Optical Coherence, Autofluorescence Lifetime imaging and Second harmonic generation (FOCALS) microscopy. This study shows that the combination of quantitative multiphoton imaging with multifunctional tags for AD offers new insights into the interaction of these tags with AD biomarkers and the theranostic mechanisms.",
keywords = "Alzheimer's disease, multimodal imaging, multiphoton microscopy, optical imaging, theranostics",
author = "Iyer, {Rishyashring R.} and Renteria, {Carlos A.} and Lingxiao Yang and Sorrells, {Janet E.} and Jaena Park and Liang Sun and Zhengxin Yu and Yiran Huang and Marina Marjanovic and Mirica, {Liviu M.} and Boppart, {Stephen A.}",
note = "This research was supported in part by grants from the National Institutes of Health (R01\u2010CA241618, R01\u2010GM114588) and the Air Force Office of Scientific Research (FA9550\u201017\u20101\u20100387). Carlos A. Renteria was supported by an NIH/NIEHS Fellowship Training Program in Endocrine, Developmental and Reproductive Toxicology (T32\u2010ES007326). Rishyashring R. Iyer was supported partly by the Mavis Future Faculty Fellows program from the Grainger College of Engineering at the University of Illinois at Urbana\u2010Champaign and partly supported by the Tissue Microenvironment Training Program funded by National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health (T32\u2010EB019944). Janet E. Sorrells was supported by a National Science Foundation Graduate Research Fellowship (DGE\u20101746047). Air Force Office of Scientific Research, Grant/Award Number: FA9550\u201017\u20101\u20100387; Graduate College, University of Illinois at Urbana\u2010Champaign, Grant/Award Number: Mavis Future Faculty Fellowship; National Cancer Institute, Grant/Award Number: R01\u2010CA241618; National Institute of Biomedical Imaging and Bioengineering, Grant/Award Number: T32\u2010EB019944; National Institute of Environmental Health Sciences, Grant/Award Number: T32\u2010ES007326; National Institute of General Medical Sciences, Grant/Award Number: R01\u2010GM114588; National Science Foundation, Grant/Award Number: DGE\u20101746047 Funding information",
year = "2022",
month = sep,
doi = "10.1002/jbio.202200105",
language = "English (US)",
volume = "15",
journal = "Journal of Biophotonics",
issn = "1864-063X",
publisher = "Wiley-VCH",
number = "9",
}