Tracking melanosomes inside a cell to study molecular motors and their interaction

Comert Kural, Anna S. Serpinskaya, Ying Hao Chou, Robert D. Goldman, Vladimir I. Gelfand, Paul R. Selvin

Research output: Contribution to journalArticle

Abstract

Cells known as melanophores contain melanosomes, which are membrane organelles filled with melanin, a dark, nonfluorescent pigment. Melanophores aggregate or disperse their melanosomes when the host needs to change its color in response to the environment (e.g., camouflage or social interactions). Melanosome transport in cultured Xenopus melanophores is mediated by myosin V, heterotrimeric kinesin-2, and cytoplasmic dynein. Here, we describe a technique for tracking individual motors of each type, both individually and in their interaction, with high spatial (≈2 nm) and temporal (≈ 1 msec) localization accuracy. This method enabled us to observe (i) stepwise movement of kinesin-2 with an average step size of 8 nm; (ii) smoother melanosome transport (with fewer pauses), in the absence of intermediate filaments (IFs); and (iii) motors of actin filaments and microtubules working on the same cargo nearly simultaneously, indicating that a diffusive step is not needed between the two systems of transport. In concert with our previous report, our results also show that dynein-driven retrograde movement occurs in 8-nm steps. Furthermore, previous studies have shown that melanosomes carried by myosin V move 35 nm in a stepwise fashion in which the step rise-times can be as long as 80 msec. We observed 35-nm myosin V steps in melanophores containing no IFs. We find that myosin V steps occur faster in the absence of IFs, indicating that the IF network physically hinders organelle transport.

Original languageEnglish (US)
Pages (from-to)5378-5382
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number13
DOIs
StatePublished - Mar 27 2007

Fingerprint

Melanosomes
Myosin Type V
Melanophores
Intermediate Filaments
Kinesin
Organelles
Cytoplasmic Dyneins
Dyneins
Melanins
Interpersonal Relations
Xenopus
Actin Cytoskeleton
Microtubules
Color
Membranes

Keywords

  • Bright-field imaging with one-nanometer accuracy (bFIONA)
  • Dynein
  • Intermediate filaments
  • Kinesin-2
  • Myosin V

ASJC Scopus subject areas

  • General

Cite this

Tracking melanosomes inside a cell to study molecular motors and their interaction. / Kural, Comert; Serpinskaya, Anna S.; Chou, Ying Hao; Goldman, Robert D.; Gelfand, Vladimir I.; Selvin, Paul R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 13, 27.03.2007, p. 5378-5382.

Research output: Contribution to journalArticle

Kural, Comert ; Serpinskaya, Anna S. ; Chou, Ying Hao ; Goldman, Robert D. ; Gelfand, Vladimir I. ; Selvin, Paul R. / Tracking melanosomes inside a cell to study molecular motors and their interaction. In: Proceedings of the National Academy of Sciences of the United States of America. 2007 ; Vol. 104, No. 13. pp. 5378-5382.
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