TY - JOUR
T1 - Towards biohythane production from biomass
T2 - Influence of operational stage on anaerobic fermentation and microbial community
AU - Si, Buchun
AU - Liu, Zhidan
AU - Zhang, Yuanhui
AU - Li, Jiaming
AU - Shen, Ruixia
AU - Zhu, Zhangbing
AU - Xing, Xinhui
N1 - Funding Information:
This work was financially supported by the National Key Technology Support Program of China ( 2014BAD02B03 ), National Natural Science Foundation of China ( 21106080 ) and the Chinese Universities Scientific Fund ( 2012RC030 ).
Publisher Copyright:
© 2015 Hydrogen Energy Publications, LLC.
PY - 2016/2/23
Y1 - 2016/2/23
N2 - Biohythane consisting of biohydrogen and biomethane via two-stage fermentation is a promising energy carrier for vehicle use. In this study, one-stage biomethane system using upflow anaerobic sludge blanket (UASB) and packed bed reactor (PBR) was shifted to the two-stage biohythane system to study the influence of operational stage. Compared with biomethane system, the biohythane process achieved higher COD removal and energy recovery. Particularly, the total COD removal in the PBR system rose significantly from 74.0 to 97.3%, corresponding to an increased energy recovery from 54.2 to 67.1%. The first-stage hydrogen fermentation had a positive effect on subsequent biomethane production in biohythane system. The analysis of microbial diversity using Illumina MiSeq sequencing showed significant changes of microorganisms in biomethane reactor, which revealed the variation of biochemical pathways. Compared to biomethane system, the relative abundance of acidogenesis bacteria was reduced in biohythane system, such as family Clostridiaceae. By contrast, the amount of acetogens (Syntrophaceae, Syntrophomonadaceae and Desulfovibrionaceae) and acetate-oxidizing bacteria (Spirochaetes) was increased. The archaea community remained stable, and mainly consisted of acetoclastic methanogens from family Methanosaetaceae. These results indicated the biomethane reactors in biohythane system had more efficient acidogenesis and acetate-utilizing microbial community.
AB - Biohythane consisting of biohydrogen and biomethane via two-stage fermentation is a promising energy carrier for vehicle use. In this study, one-stage biomethane system using upflow anaerobic sludge blanket (UASB) and packed bed reactor (PBR) was shifted to the two-stage biohythane system to study the influence of operational stage. Compared with biomethane system, the biohythane process achieved higher COD removal and energy recovery. Particularly, the total COD removal in the PBR system rose significantly from 74.0 to 97.3%, corresponding to an increased energy recovery from 54.2 to 67.1%. The first-stage hydrogen fermentation had a positive effect on subsequent biomethane production in biohythane system. The analysis of microbial diversity using Illumina MiSeq sequencing showed significant changes of microorganisms in biomethane reactor, which revealed the variation of biochemical pathways. Compared to biomethane system, the relative abundance of acidogenesis bacteria was reduced in biohythane system, such as family Clostridiaceae. By contrast, the amount of acetogens (Syntrophaceae, Syntrophomonadaceae and Desulfovibrionaceae) and acetate-oxidizing bacteria (Spirochaetes) was increased. The archaea community remained stable, and mainly consisted of acetoclastic methanogens from family Methanosaetaceae. These results indicated the biomethane reactors in biohythane system had more efficient acidogenesis and acetate-utilizing microbial community.
KW - Biohydrogen
KW - Biohythane
KW - Microbial diversity
KW - Operational stage
KW - Two-stage
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U2 - 10.1016/j.ijhydene.2015.06.045
DO - 10.1016/j.ijhydene.2015.06.045
M3 - Article
AN - SCOPUS:84961054098
VL - 41
SP - 4429
EP - 4438
JO - International Journal of Hydrogen Energy
JF - International Journal of Hydrogen Energy
SN - 0360-3199
IS - 7
ER -