Towards an immunosense vaccine to prevent toxoplasmosis: Protective Toxoplasma gondii epitopes restricted by HLA-A*0201

Hua Cong, Ernest J. Mui, William H. Witola, John Sidney, Jeff Alexander, Alessandro Sette, Ajesh Maewal, Rima McLeod

Research output: Research - peer-reviewArticle

Abstract

The ideal vaccine to protect against toxoplasmosis in humans would include antigens that elicit a protective T helper cell type 1 immune response, and generate long-lived IFN-γ-producing CD8+ T cells. Herein, we utilized a predictive algorithm to identify candidate HLA-A02 supertype epitopes from Toxoplasma gondii proteins. Thirteen peptides elicited production of IFN-γ from PBMC of HLA-A02 supertype persons seropositive for T. gondii infection but not from seronegative controls. These peptides displayed high-affinity binding to HLA-A02 proteins. Immunization of HLA-A*0201 transgenic mice with these pooled peptides, with a universal CD4+ epitope peptide called PADRE, formulated with adjuvant GLA-SE, induced CD8+ T cell IFN-γ production and protected against parasite challenge. Peptides identified in this study provide candidates for inclusion in immunosense epitope-based vaccines.

LanguageEnglish (US)
Pages754-762
Number of pages9
JournalVaccine
Volume29
Issue number4
DOIs
StatePublished - Jan 17 2011
Externally publishedYes

Fingerprint

Toxoplasmosis
Toxoplasma
Epitopes
Vaccines
Peptides
HLA-A*02:01 antigen
toxoplasmosis
Toxoplasma gondii
epitopes
peptides
vaccines
varespladib methyl
T-lymphocytes
T-Lymphocytes
Proteins
proteins
Th1 Cells
Transgenic Mice
Immunization
Parasites

Keywords

  • HLA-A2 epitope
  • Toxoplasma gondii
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Towards an immunosense vaccine to prevent toxoplasmosis : Protective Toxoplasma gondii epitopes restricted by HLA-A*0201. / Cong, Hua; Mui, Ernest J.; Witola, William H.; Sidney, John; Alexander, Jeff; Sette, Alessandro; Maewal, Ajesh; McLeod, Rima.

In: Vaccine, Vol. 29, No. 4, 17.01.2011, p. 754-762.

Research output: Research - peer-reviewArticle

Cong, H, Mui, EJ, Witola, WH, Sidney, J, Alexander, J, Sette, A, Maewal, A & McLeod, R 2011, 'Towards an immunosense vaccine to prevent toxoplasmosis: Protective Toxoplasma gondii epitopes restricted by HLA-A*0201' Vaccine, vol 29, no. 4, pp. 754-762. DOI: 10.1016/j.vaccine.2010.11.015
Cong, Hua ; Mui, Ernest J. ; Witola, William H. ; Sidney, John ; Alexander, Jeff ; Sette, Alessandro ; Maewal, Ajesh ; McLeod, Rima. / Towards an immunosense vaccine to prevent toxoplasmosis : Protective Toxoplasma gondii epitopes restricted by HLA-A*0201. In: Vaccine. 2011 ; Vol. 29, No. 4. pp. 754-762
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