Towards an immunosense vaccine to prevent toxoplasmosis: Protective Toxoplasma gondii epitopes restricted by HLA-A*0201

Hua Cong, Ernest J. Mui, William H. Witola, John Sidney, Jeff Alexander, Alessandro Sette, Ajesh Maewal, Rima McLeod

Research output: Contribution to journalArticlepeer-review

Abstract

The ideal vaccine to protect against toxoplasmosis in humans would include antigens that elicit a protective T helper cell type 1 immune response, and generate long-lived IFN-γ-producing CD8+ T cells. Herein, we utilized a predictive algorithm to identify candidate HLA-A02 supertype epitopes from Toxoplasma gondii proteins. Thirteen peptides elicited production of IFN-γ from PBMC of HLA-A02 supertype persons seropositive for T. gondii infection but not from seronegative controls. These peptides displayed high-affinity binding to HLA-A02 proteins. Immunization of HLA-A*0201 transgenic mice with these pooled peptides, with a universal CD4+ epitope peptide called PADRE, formulated with adjuvant GLA-SE, induced CD8+ T cell IFN-γ production and protected against parasite challenge. Peptides identified in this study provide candidates for inclusion in immunosense epitope-based vaccines.

Original languageEnglish (US)
Pages (from-to)754-762
Number of pages9
JournalVaccine
Volume29
Issue number4
DOIs
StatePublished - Jan 17 2011

Keywords

  • HLA-A2 epitope
  • Toxoplasma gondii
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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