Total parenteral nutrition alters molecular and cellular indices of intestinal inflammation in neonatal piglets

Research output: Contribution to journalArticle

Abstract

Background: The adverse effects of TPN on systemic immunity are well- documented; however, the impact of IV feeding on neonatal intestinal immunity is unknown. Methods: A piglet TPN model was used to compare immune cell composition within the intestinal epithelium and lamina propria of parenterally and orally fed piglets. Results: Small intestinal weight of piglets maintained intravenously was reduced 50% after 7 days. Intestinal atrophy in piglets fed parenterally was evidenced by decreased width of intestinal villi and colon cuffs and reduced intestinal crypt depth. The numbers of CD4+ and CD8+ T lymphocytes were threefold greater within the lamina propria of jejunal and ileal villi of piglets supported intravenously. Inverse correlations were observed between villus height or width and T- lymphocyte numbers (r = -.80; p < .05). Major histocompatibility complex class II mRNA expression, an indicator of localized inflammation, was increased in the ileum and colon of piglets receiving parenteral nutrition. Goblet cell numbers were twofold greater in jejunal and ileal villi, and mast cells were more abundant in the colon of piglets fed parenterally. Furthermore, jejunal T-lymphocyte numbers were correlated with goblet cell numbers (r = .80; p = .01). Conclusions: These data identify molecular and cellular indices of intestinal inflammation that are responsive to IV feeding in neonates and provide a novel framework to investigate mechanisms underlying gut atrophy during TPN.

Original languageEnglish (US)
Pages (from-to)337-344
Number of pages8
JournalJournal of Parenteral and Enteral Nutrition
Volume23
Issue number6 SUPPL.
DOIs
StatePublished - Jan 1 1999

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Total Parenteral Nutrition
Colon
Goblet Cells
Lymphocyte Count
Inflammation
T-Lymphocytes
Atrophy
Immunity
Mucous Membrane
Cell Count
Parenteral Nutrition
Intestinal Mucosa
Major Histocompatibility Complex
Ileum
Mast Cells
Weights and Measures
Messenger RNA

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

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title = "Total parenteral nutrition alters molecular and cellular indices of intestinal inflammation in neonatal piglets",
abstract = "Background: The adverse effects of TPN on systemic immunity are well- documented; however, the impact of IV feeding on neonatal intestinal immunity is unknown. Methods: A piglet TPN model was used to compare immune cell composition within the intestinal epithelium and lamina propria of parenterally and orally fed piglets. Results: Small intestinal weight of piglets maintained intravenously was reduced 50{\%} after 7 days. Intestinal atrophy in piglets fed parenterally was evidenced by decreased width of intestinal villi and colon cuffs and reduced intestinal crypt depth. The numbers of CD4+ and CD8+ T lymphocytes were threefold greater within the lamina propria of jejunal and ileal villi of piglets supported intravenously. Inverse correlations were observed between villus height or width and T- lymphocyte numbers (r = -.80; p < .05). Major histocompatibility complex class II mRNA expression, an indicator of localized inflammation, was increased in the ileum and colon of piglets receiving parenteral nutrition. Goblet cell numbers were twofold greater in jejunal and ileal villi, and mast cells were more abundant in the colon of piglets fed parenterally. Furthermore, jejunal T-lymphocyte numbers were correlated with goblet cell numbers (r = .80; p = .01). Conclusions: These data identify molecular and cellular indices of intestinal inflammation that are responsive to IV feeding in neonates and provide a novel framework to investigate mechanisms underlying gut atrophy during TPN.",
author = "Deshanie Ganessunker and Gaskins, {H Rex} and Zuckermann, {Federico A} and Donovan, {Sharon M}",
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T1 - Total parenteral nutrition alters molecular and cellular indices of intestinal inflammation in neonatal piglets

AU - Ganessunker, Deshanie

AU - Gaskins, H Rex

AU - Zuckermann, Federico A

AU - Donovan, Sharon M

PY - 1999/1/1

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N2 - Background: The adverse effects of TPN on systemic immunity are well- documented; however, the impact of IV feeding on neonatal intestinal immunity is unknown. Methods: A piglet TPN model was used to compare immune cell composition within the intestinal epithelium and lamina propria of parenterally and orally fed piglets. Results: Small intestinal weight of piglets maintained intravenously was reduced 50% after 7 days. Intestinal atrophy in piglets fed parenterally was evidenced by decreased width of intestinal villi and colon cuffs and reduced intestinal crypt depth. The numbers of CD4+ and CD8+ T lymphocytes were threefold greater within the lamina propria of jejunal and ileal villi of piglets supported intravenously. Inverse correlations were observed between villus height or width and T- lymphocyte numbers (r = -.80; p < .05). Major histocompatibility complex class II mRNA expression, an indicator of localized inflammation, was increased in the ileum and colon of piglets receiving parenteral nutrition. Goblet cell numbers were twofold greater in jejunal and ileal villi, and mast cells were more abundant in the colon of piglets fed parenterally. Furthermore, jejunal T-lymphocyte numbers were correlated with goblet cell numbers (r = .80; p = .01). Conclusions: These data identify molecular and cellular indices of intestinal inflammation that are responsive to IV feeding in neonates and provide a novel framework to investigate mechanisms underlying gut atrophy during TPN.

AB - Background: The adverse effects of TPN on systemic immunity are well- documented; however, the impact of IV feeding on neonatal intestinal immunity is unknown. Methods: A piglet TPN model was used to compare immune cell composition within the intestinal epithelium and lamina propria of parenterally and orally fed piglets. Results: Small intestinal weight of piglets maintained intravenously was reduced 50% after 7 days. Intestinal atrophy in piglets fed parenterally was evidenced by decreased width of intestinal villi and colon cuffs and reduced intestinal crypt depth. The numbers of CD4+ and CD8+ T lymphocytes were threefold greater within the lamina propria of jejunal and ileal villi of piglets supported intravenously. Inverse correlations were observed between villus height or width and T- lymphocyte numbers (r = -.80; p < .05). Major histocompatibility complex class II mRNA expression, an indicator of localized inflammation, was increased in the ileum and colon of piglets receiving parenteral nutrition. Goblet cell numbers were twofold greater in jejunal and ileal villi, and mast cells were more abundant in the colon of piglets fed parenterally. Furthermore, jejunal T-lymphocyte numbers were correlated with goblet cell numbers (r = .80; p = .01). Conclusions: These data identify molecular and cellular indices of intestinal inflammation that are responsive to IV feeding in neonates and provide a novel framework to investigate mechanisms underlying gut atrophy during TPN.

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