Total internal reflection fluorescence of molecular beacons in a multiplexed microfluidic device

Benjamin R. Schudel, Melikhan Tanyeri, Charles M. Schroeder, Paul J.A. Kenis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Molecular beacons can be used as an ultra-sensitive means of DNA detection, with discrimination capability down to a single base pair mismatch. Assays that utilize these beacons are often performed in 96- or 386-well microplates. To vastly increase the benefits of molecular beacon technology, we present here a combinatorial microfluidic network with surface-tethered molecular beacons for highly sensitive identification of oligomer targets. Total internal reflection fluorescence microscopy (TIRF-M) is used for characterization of binding events in a multiplexed microfluidic array of sub-nanoliter wells.

Original languageEnglish (US)
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages36-38
Number of pages3
ISBN (Print)9780979806421
StatePublished - Jan 1 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: Nov 1 2009Nov 5 2009

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Country/TerritoryKorea, Republic of
CityJeju
Period11/1/0911/5/09

Keywords

  • Molecular beacons
  • Multiplexing
  • TIRF
  • Virus identification

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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