Total internal reflection fluorescence of molecular beacons in a multiplexed microfluidic device

Benjamin R. Schudel, Melikhan Tanyeri, Charles M. Schroeder, Paul J.A. Kenis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Molecular beacons can be used as an ultra-sensitive means of DNA detection, with discrimination capability down to a single base pair mismatch. Assays that utilize these beacons are often performed in 96- or 386-well microplates. To vastly increase the benefits of molecular beacon technology, we present here a combinatorial microfluidic network with surface-tethered molecular beacons for highly sensitive identification of oligomer targets. Total internal reflection fluorescence microscopy (TIRF-M) is used for characterization of binding events in a multiplexed microfluidic array of sub-nanoliter wells.

Original languageEnglish (US)
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages36-38
Number of pages3
ISBN (Print)9780979806421
StatePublished - Jan 1 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: Nov 1 2009Nov 5 2009

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period11/1/0911/5/09

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Keywords

  • Molecular beacons
  • Multiplexing
  • TIRF
  • Virus identification

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Schudel, B. R., Tanyeri, M., Schroeder, C. M., & Kenis, P. J. A. (2009). Total internal reflection fluorescence of molecular beacons in a multiplexed microfluidic device. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 36-38). (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.