Topography and refractometry of sperm cells using spatial light interference microscopy

Lina Liu, Mikhail E. Kandel, Marcello Rubessa, Sierra Schreiber, Mathew B. Wheeler, Gabriel Popescu

Research output: Contribution to journalArticle

Abstract

Characterization of spermatozoon viability is a common test in treating infertility. Recently, it has been shown that label-free, phase-sensitive imaging can provide a valuable alternative for this type of assay. We employ spatial light interference microscopy (SLIM) to perform high-accuracy single-cell phase imaging and decouple the average thickness and refractive index information for the population. This procedure was enabled by quantitative-phase imaging cells on media of two different refractive indices and using a numerical tool to remove the curvature from the cell tails. This way, we achieved ensemble averaging of topography and refractometry of 100 cells in each of the two groups. The results show that the thickness profile of the cell tail goes down to 150 nm and the refractive index can reach values of 1.6 close to the head.

Original languageEnglish (US)
Article number025003
JournalJournal of biomedical optics
Volume23
Issue number2
DOIs
StatePublished - Feb 1 2018

Keywords

  • refractive index
  • refractometry
  • spatial light interference microscopy
  • sperm cell
  • topography

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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