Tissue-specific expression and localization of safener-induced glutathione S-transferase proteins in Triticum tauschii

Dean E. Riechers, Qin Zhang, Fangxiu Xu, Kevin C. Vaughn

Research output: Contribution to journalArticlepeer-review


Glutathione S-transferase (GST; EC gene expression was examined in the coleoptile and new leaf tissue of etiolated shoots of the diploid wheat species Triticum tauschii (Coss.) Schmal., which is considered to be a progenitor and the D-genome donor to cultivated, hexaploid bread wheat Triticum aestivum L. GST expression (mRNA, protein, and enzyme activity with a herbicide substrate) in these shoot tissues was examined in response to herbicide safener treatment. Two different antibody probes, raised against the same safener-inducible GST protein (TtGSTU1) but differing in their specificity, were utilized to determine tissue distribution and subcellular localization of GST proteins in etiolated shoots. GST transcripts, immunoreactive GST proteins, and herbicide-metabolizing activity were all highest in the coleoptile of etiolated, safener-treated T. tauschii shoots. Anti-GST immunolabeling was strongest in the outer epidermal and adjoining sub-epidermal cells in both coleoptiles and new leaves following safener treatment. Our data indicate that safeners protect grass crops from herbicide injury by dramatically inducing the expression of GST proteins in the outer cell layers of the coleoptile, which prevents the herbicide from reaching the sensitive new leaves of etiolated shoots as they emerge from the soil.

Original languageEnglish (US)
Pages (from-to)831-840
Number of pages10
Issue number5
StatePublished - Sep 1 2003


  • Coleoptile
  • Detoxification
  • Gene expression
  • Herbicide safeners
  • Protein localization
  • Triticum

ASJC Scopus subject areas

  • Genetics
  • Plant Science


Dive into the research topics of 'Tissue-specific expression and localization of safener-induced glutathione S-transferase proteins in Triticum tauschii'. Together they form a unique fingerprint.

Cite this