Tissue factor localization in non-human primate cerebral tissue

G. J. Del Zoppo, J. Q. Yu, B. R. Copeland, W. S. Thomas, J. Schneiderman, J. H. Morrissey

Research output: Contribution to journalArticle

Abstract

Tissue factor (TF), the principal procoagulant of human brain, resides in specific regions of the non-human primate central nervous system. Immunohistochemical studies employing murine anti-human TF monoclonal antibodies (MoAbs) detected TF antigen in the cortex, basal ganglia, cerebellum, and cervical spinal cord in three normal baboon subjects. Although significantly less prominent than human cortical gray matter, a distinct partition of TF in gray matter >white matter was noted. The gray matter predilection of TF was confirmed in primate temporal and parietal lobe cortex by both sandwich ELISA and one-stage coagulation assay. Variation in the relative quantity of TF antigen was observed by ELISA among the three subjects studied. Procoagulant activity followed the pattern of TF antigen (cortical gray matter >basal ganglia ≥cerebellum >cortical white matter), and was 96.5-98.5% inhibitable by a function inhibiting antihuman TF MoAb combination. TF antigen was associated with the microvasculature of all cerebral tissues studied, and spared capillaries most selectively in the cerebral cortex, basal ganglia, and cerebellum. These findings suggest a highly specific ordering of TF antigen and related procoagulant activity in the central nervous system of the baboon, confined primarily to gray matter parenchyma, and to the non-capillary microvasculature.

Original languageEnglish (US)
Pages (from-to)642-647
Number of pages6
JournalThrombosis and Haemostasis
Volume68
Issue number6
StatePublished - Jan 1 1992

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Thromboplastin
Primates
Antigens
Basal Ganglia
Cerebellum
Parietal Lobe
Papio
Microvessels
Central Nervous System
Enzyme-Linked Immunosorbent Assay
Temporal Lobe
Cerebral Cortex
Monoclonal Antibodies
Gray Matter
Brain

ASJC Scopus subject areas

  • Hematology

Cite this

Del Zoppo, G. J., Yu, J. Q., Copeland, B. R., Thomas, W. S., Schneiderman, J., & Morrissey, J. H. (1992). Tissue factor localization in non-human primate cerebral tissue. Thrombosis and Haemostasis, 68(6), 642-647.

Tissue factor localization in non-human primate cerebral tissue. / Del Zoppo, G. J.; Yu, J. Q.; Copeland, B. R.; Thomas, W. S.; Schneiderman, J.; Morrissey, J. H.

In: Thrombosis and Haemostasis, Vol. 68, No. 6, 01.01.1992, p. 642-647.

Research output: Contribution to journalArticle

Del Zoppo, GJ, Yu, JQ, Copeland, BR, Thomas, WS, Schneiderman, J & Morrissey, JH 1992, 'Tissue factor localization in non-human primate cerebral tissue', Thrombosis and Haemostasis, vol. 68, no. 6, pp. 642-647.
Del Zoppo GJ, Yu JQ, Copeland BR, Thomas WS, Schneiderman J, Morrissey JH. Tissue factor localization in non-human primate cerebral tissue. Thrombosis and Haemostasis. 1992 Jan 1;68(6):642-647.
Del Zoppo, G. J. ; Yu, J. Q. ; Copeland, B. R. ; Thomas, W. S. ; Schneiderman, J. ; Morrissey, J. H. / Tissue factor localization in non-human primate cerebral tissue. In: Thrombosis and Haemostasis. 1992 ; Vol. 68, No. 6. pp. 642-647.
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